Abstract 19866: Investigating Lipidomics of Patient Specific iPSC Derived Hepatocytes

Introduction: Hepatocyte-like cells (HLCs) differentiated from human induced pluripotent stem cells (hiPSCs) offer an alternative model for primary human hepatocytes (PHHs) when analyzing the functionality and lipid secretion properties of liver is desired in a patient-specific manner. The detailed lipid profile of HLCs is, however, unknown. Here, we aim to develop an in vitro model of hepatocytes derived from patients with coronary artery disease (CAD) in order to study their lipidomics. Method: hiPSCs were derived from skin biopsies and then differentiated into functional HLCs. Five hepatic differentiation methods were tested to find the most efficient and robust protocol. During the entire differentiation process from hiPSCs to HLCs, we monitored the detailed quantification of alteration in cellular lipidome and gene expression profiling of a set of 16 lipid metabolism-related genes using mass spectrometry and qPCR respectively. Additionally, biochemical (TG, albumin, and urea secretion), functional (L...