Establishment of Genetic Transformation System of Cucumber 'New Jinchun 4'

The genetic transformation system of cucumber 'New Jinchun 4' mediated by Agrobacterium tumefaciens were studied.The cucumber leaves as explants were pre-cultured on MS+1.0 mg L-1 6-BA+0.15 mg L-1 IAA for 2 days.After infected by Agrobacterium(OD600=0.8,pH=5.7) for 8 min supplemented with 150 μmol/L acetosyringone(AS),the explants were co-cultured on MS + 1.0 mg L-1 6-BA + 0.15 mg L-1 IAA + 500 mg L-1 Carb + 40 mg L-1 Hyg for 2 days.The hpt gene were transformed successfully to regenerated plantlets by gus instantaneous expression and PCR test.