Thyroid microsomal antibody detection reagent kit as well as preparation method and application thereof

The invention relates to a chemical luminescent immunodetection thyroid microsomal antibody detection reagent kit and a preparation method of the reagent kit. The reagent kit comprises a protein A antigen labeled with a tracer marker, or an anti-human IgG and anti-human IgM labeled with the tracer marker, a thyroid microsomal antigen coated with magnetic spheres, or a junction complex of a protein carrier and the thyroid microsomal antigen coated with the magnetic spheres. The invention also provides a method for detecting the concentration of the thyroid microsomal antibody. When the reagent kit provided by the invention is used for detecting the concentration of the thyroid microsomal antibody, the enzymatic reaction is not involved, and the bridging of enzyme is not needed, so that the reagent is long in preservation time and stable; the detection also can be carried out by virtue of a full-automatic chemical luminescent method, so that the operation time is shortened, the manmade operation error is reduced, and the sensitivity and the accuracy in detection can be improved by utilizing the specificity of the chemical tracer marker.