Experimental study on the transplantation of bone marrow-derived neural stem cell for the treatment of temporal lobe epilepsy in rats

Objective To explore the integrating and repairing effect of bone marrow-derived neural stem cell after its transplantation into the hippocampi of KA rats,and to provide theoretical basis of stem cell for the treatment of temporal lobe epilepsy(TLE).Method The bone marrow stromal cells(BMSCs) of rats were isolated,and under specific condition the BMSCs were cultured to induce and differentiate into neural stem cells(NSCs).And the NSCs were labeled with Feridex.Then the rat models of temporal lobe epilepsy were established,and the NSCs were auto-transplanted into the hippocampi of KA rats.The changes of EEG,pathology and MRI were analyzed 1,2,4,8,16 weeks after the transplantation respectively.Result Compared with the non-graft group,the EEG magnitude of the graft group decreased significantly,even more than 40%.There was significant difference in the number of hippocampal CA3 pyramid cells between the non-graft and graft group(P0.01).And there was also significant difference in Timm staining of the injured hippocampus between the non-graft and graft group(P0.01).MRI testing showed that low signal region localized 1 and 2 weeks after the transplantation,while it spread more widely,and with time going on the low signal slowly increased 4,8,16 weeks after the transplantation.Conclusion When the bone marrow-derived NSCs are transplanted into the KA rats,they can integrate into the host and have a remarkable repairing effect on the hippocampus.But its actual principle still need to be researched on.