Determination of Total Polychlorinated n-Alkane Concentration in Biota by Electron Ionization-MS/MS

Electron ionization (EI) tandem mass spectrometry (MS/MS) allowed the fast determination of the total concentration of short- and medium-chained polychlorinated n-alkanes (PCAs) in biota. EI fragment ions common to all PCAs could be identified. Collision-induced dissociations (CIDs) were carried out by ion trap and triple quadrupole EI-MS/MS. CIDs of m/z 91 → 53 (limit of detection (LOD) 0.15 ng/μL), 102 → 65 (LOD = 0.2 ng/μL), and 102 → 67 (LOD = 0.1 ng/μL) were applied for the determination of the total short- and medium-chain PCA concentration in pooled fish liver samples (North Sea dab, cod, flounder) from the North Sea and from the Baltic Sea using both MS technologies. Total PCA concentrations were in the range of 88−607 ng/g. Accuracy was controlled with spiked samples and deviated not more than 15% from expected values.