Prussian blue nanocubes decorated three-dimensional silver nanowires network for high-performance electrochemical biosensing

Abstract High sensitivity and selectivity play critical roles in practical applications of glucose biosensors. In this study, we reported a novel glucose biosensor based on three-dimensional (3D) structured biosensing film, which consisted of silver nanowires (AgNWs) network and Prussian blue nanocubes (PBNCs). 3D AgNWs network was successfully built on the electrode surface with assistance of self-assembled monolayers (SAMs), while PBNCs were in situ prepared on the network through aerosol deposition approach and glucose oxidase (GOx) was immobilized onto the surface of PBNCs/AgNWs film with glutaraldehyde cross-linking method. Owing to an enhanced surface area, high conductivity and synergistic electrocatalytic activity of fabricated PBNCs/AgNWs film, an ultrasensitive glucose biosensor with high selectivity was constructed. As a result, the as-prepared biosensor exhibited an ultrahigh sensitivity of 359.0 mA M −1  cm −2 with a linear range from 0.01 to 0.9 mM, an outstanding anti-interference ability at a low potential of −0.05 V, as well as excellent stability and reproducibility. It is believed that the proposed 3D sensing film would have widespread applications in biological sensing and quantitative biochemical analysis.