Methods Cell culture apparatus and cell culture

A cell culture device having a culture area of ​​culturing stem cells or progenitor cells to form tissue, and oxygen adjusting unit for adjusting the oxygen supply amount of the culture area, and a control unit for controlling the oxygen adjusting unit, the among the culture period cell, in the second period in which the stem cells or progenitor cells contain a first oxygen supply amount in the first period within the period of self-replication, the period in which the stem cells or progenitor cells differentiate, the a second oxygen supply of more oxygen supply than the first oxygen supply, the control, based on the degree of proliferation by self-renewal of said stem cells or progenitor cells, the said first oxygen supply to change to the second oxygen supply, a cell culture apparatus characterized by comprising a control unit for controlling the oxygen regulating unit.