MZF1-N terminal antibody prepared through GST expression system and applications thereof

The invention belongs to the technical field of biology engineering, and relates to an MZF1-N terminal antibody prepared through a GST expression system and applications thereof. By utilizing a gene engineering technology, an MZF1 gene is cloned to a prokaryotic expression vector, after enzymatic detection, the recombinant plasmid is converted to escherichia coli, and the escherichia coli is subjected to IPTG induction to generate GST-MZF1 fusion protein. Purified fusion protein is used to immunize New Zealand rabbits to prepare antiserum, and then Protein A/G is used to purify the antiserum. The titer and specificity of antibody are detected by ELISA and Western blot. The titer and specificity of the MZF1 polyclonal antibody are good, and the MZF1 polyclonal antibody can be used to detect MZF1 protein in cells and is capable of effectively detecting the expression difference of MZF1 protein in different cells, so the MZF1 polyclonal antibody has an important meaning for the researches on the functions of MZF1 protein.