Aging and Atherosclerosis Impair Hematopoietic Stem Cell Function

Background: Stem cell function is compromised with aging, especially when a cardiovascular disease is associated. The aim of this study was to evaluate how aging and atherosclerosis affect bone marrow hematopoietic stem cell (HSC) function in mice. Methods: Male mice were divided into C57 and apoE-/- (2, 12 and 24-month-old) groups. Oxidative stress was assessed by 2,7-dichlorofluorescein (DCF) by flow cytometer. For apoptosis detection, HSC were incubated with Annexin V-FITC and propidium iodide (PI). Plasma inflammation markers, IL-6 and MCP-1, were measured. Also, senescence was evaluated by acridine orange protocol. Lipid profile and aortic lipid deposition were also analyzed. Data are mean±SEM. Statistical analysis was performed with two way ANOVA test and Tukey pos hoc. Results: Aging and atherosclerosis increased ROS levels and apoptosis in HSC. Senescence was also increased with aging and atherosclerosis, especially in 24-month-old mice. Atherosclerotic adult and aged animals showed higher levels ...