SPECTROPHOTOMETRIC DETERMINATION OF LEVETIRACETAM USING 2, 4-DNP IN PHARMACEUTICAL DOSAGE FORM

Article history A simple, extraction free spectrophotometric method for the quantitative estimation of levetiracetam (LEV) in bulk drugs and pharmaceutical formulations (tablets) has been developed. The method is based on the oxidation of 2, 4- dinitrophenylhydrazine (2, 4-DNP) and coupling of the oxidized product with drugs to give intensely colored chromogen. Levetiracetam at its λmax 455 nm shows linearity in the concentration range of 30-130 µg mL -1 . The relative standard deviation for this method is 0.422%. Linear relationship with good correlation coefficients (0.995-0.996) was found between absorbance and the corresponding concentrations of the drug. The reliability and performance of the proposed method was validated statistically the percentage recovery ranged from 99.88 and 100.2. The results of analysis for this method have been validated statistically and by recovery studies.