Genetically encoded fluorescent sensors reveal dynamic regulation of NADPH metabolism

Rongkun Tao,Yuzheng Zhao,Huanyu Chu,Aoxue Wang,Jiahuan Zhu,Xianjun Chen,Yejun Zou,Mei Shi,Renmei Liu,Ni Su,Jiulin Du,Hai-Meng Zhou,Linyong Zhu,Xuhong Qian,Haiyan Liu,Joseph Loscalzo,Yi Yang

Genetically encoded fluorescent sensors reveal dynamic regulation of NADPH metabolism

2017

Rongkun Tao
Yuzheng Zhao
Huanyu Chu
Aoxue Wang
Jiahuan Zhu
Xianjun Chen
Yejun Zou
Mei Shi
Renmei Liu
Ni Su
Jiulin Du
Hai-Meng Zhou
Linyong Zhu
Xuhong Qian
Haiyan Liu
Joseph Loscalzo
Yi Yang

Genetically encoded iNap sensors allow imaging of NADPH with high spatiotemporal resolution in living systems. The iNaps cover physiologically relevant NADPH concentrations and are demonstrated in mammalian cells and live zebrafish.

Keywords:

Molecular biology
NADPH metabolism
Bimolecular fluorescence complementation
Cellular imaging
Zebrafish
Biology
Protein design
Fluorescence-lifetime imaging microscopy
Cell biology
Biochemistry
Fluorescence
spatiotemporal resolution

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