Transgene structures of marker-free transgenic Bt rice plants

Abstract A less simple approach developed for generation of marker-free transgenic plants is to select transformants without the use of selective marker genes. Some results about development of marker-free transgenic plants were obtained using a non-selective approach in several crops such as rice, potato and tobacco. However, the study did not provide evidence on detailed characterization of introduced gene on genome, a critical step for confirming the stable integration and transmission of a foreign gene. In this study, we evaluated structure and integration sites of transgene (m Cry1Ac ) in the transgenic Bt rice plants which were made via conventional Agrobacterium -mediated transformation by non-selective method. Structure and integration sites of transgene in these transgenic plants had similar fashion as those recovered under selection. Keywords Non-selective, Marker-free transgenic rice, Agrobacterium -mediated transformation, Transgene, integration site 서 론 선발마커유전자(Selective marker gene)는 목표유전자와 연결하여, 도입유전자가 형질전환 된 식물체를 선택적으로 선발하는데 사용된다. 통상적으로