Experimental investigation of peripheral nerve repaired by neural stem cells from allo-and self-body bone marrow

[Objective] To investigate the repairing effect of grafted neural stem cells from allo-and self-body marrow on the peripheral nerve and discuss if they can survive in the form of differentiated neural cells.[Method]The bone marrow stroma-cells from New Zealand rabbits were cultured and induced into stem cells. BrdU and SABC methods were adopted to mark and detect them. The allo-body grafting group, self-body grafting group and control group were made respectively from random selection.[Result] Nerve fibers of both allo-and self-body grafting area showed the array of ripple, which was the same shape as the normal fibers except for lower density. Node of ranvier of myelinated nerve fibers and mucus matrix between the inter-space of nerve fibers and little fibroblast were seen. The nerve fibers of none-grafting area were rarefied, discontinuous and many disruptions were found. The walk sequence of nerve fiber of allo-grafting was more disorder than that of self-grafting. Differentiated cells were not found in both allo-and self-grafting area. [Conclusion]Both neural stem cells from allo-and self-body bone marrow are helpful to repair peripheral nerve, the living condition of allo-body stem cells is worse than that of self-dody stem cells. Neither of them survive in differentiated form.