Determining method for total flavone content of blumea balsamifera

The invention discloses a determining method for the total flavone content of blumea balsamifera or blumea balsamifera extractive or materials having the same total flavone with the blumea balsamifera, relating to an ultraviolet-visible spectrophotometry. The determining method comprises the following steps of: preparing a gradient standard solution by using rutin or blumeatin (3, 3', 5, 7-tetramethyl-4'-methoxy flavanone) reference material, preparing a solution to be tested by using a blumea balsamifera medical material or the blumea balsamifera extractive, directly determining the absorbance of the gradient standard solution under the wavelength of 271nm, and establishing a standard curve; and determining the absorbance of the solution to be tested again, finding out the concentration of the solution to be tested from the standard curve, and finally working out the total flavone content of the blumea balsamifera medical material or extractive according to the dilution multiple of solution preparation. According to the determining method, an isoabsorptive point is adopted for absorbing wavelengths to determine the total contents of the two types of materials with different ultraviolet absorptions; and the determining method has the advantages of strong pertinence, accurate and reliable results, good accuracy, stability and repeatability, high degree of accuracy, easiness in control, simpleness and convenience in operation and can be used for the quality control of the blumea balsamifera medical material or extractive.