Co-effect of decorin and interleukin-24 on human PBMCs in vitro

Objective To investigate the co-effect of decorin (DCN) and interleukin-24 (IL-24) on proliferation and Interferon-γ (IFN-γ) secretion of human peripheral blood mononuclear cells (PBMCs). Methods Recombinant plasmid pcDNA3. 1 (+)-DCN and pcDNA3. 1 (+)-IL-24 were constructed and transfected into PBMCs by liposome. After transfected with different plasmids, the PBMCs were divided into 6 groups;blank control group, Lipofectamine TM group, empty vector group, DCN group, IL-24 group, DCN and IL-24 group. PBMC proliferation was determined by methyl thiazolyl tetrazolium assay. IFN-7 expression in the supernatant was detected by ELISA. Flow cytometry was used to determine the surface expression of programmed death-1 (PD-1) on PBMCs. Statistical analysis was made using LSD-t test. Results The recombinant plasmid pcDNA3. 1 (+)-IL-24 was constructed successfully. PBMC proliferation and IFN-7 secretion were significantly higher in DCN and IL-24 group, and the expression of PD-1 was also upregulated obviously. Conclusion The combination of DCN and IL-24 could promote PBMC proliferation and strengthen the function of PBMCs in vitro. Key words: Proteoglycans; Interleukins; Monocytes; Gene therapy; Interferon type Ⅱ; Programmed death-1