STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DOMPERIDONE AND LAFUTIDINE IN BULK AND THE PHARMACEUTICAL DOSAGE FORM Research Article

A stability indicating RP-HPLC method was developed and validated for the simultaneous estimation of Domperidone and Lafutidine in bulk and pharmaceutical dosage form. Chromatography was carried on Thermo Hypersil BDS C 18 (250mm x 4.6i.d, 5µm) column with mobile phase comprising of dipotassium hydrogen phosphate (0.1M) buffer and methanol in the ratio 60:40 v/v. The flow rate was adjusted to 1.0ml/min with UV detection at 280nm. The retention times of Domperidone, Lafutidine were found to be 1.813 min, 8.949 min respectively. The different analytical parameters such as accuracy, linearity, precision, robustness, limit of detection(LOD), limit of quantification (LOQ) were determined according to the International Conference on Harmonization (ICH) Q2B guidelines. The detector response was linear in the range of 180-540µg/ml, 60-180µg/ml for Domperidone and Lafutidine, respectively. In the linearity study, the regression equation and coefficient of correlation for Domperidone and Lafutidine, were found to be (y =16040x+1400, r2 = 1),(y = 22541x+3490, r2