Abstract 20389: Transendocardial Stem Cell Injection Site Tissue Viability Predicts Scar Size Reduction in Myocardial Segmental Analysis in a Swine Model of Ischemic Cardiomyopathy

Background: Transendocardial Stem Cell Injections (TESI) decrease scar size. We hypothesized that there is greater scar size reduction when TESI occurs in infarct-border zone (IZ/BZ) with more viability (>6mV Unipolar voltage) compared to segments with less viability (<6mV), and scar size reduction is enhanced when TESI occurs with premature ventricular contractions (PVCs). Methods: Gottingen mini-swine (n=28) underwent 150-minute occlusion of the LAD coronary artery followed by reperfusion. Cardiac magnetic resonance imaging (CMR) was obtained before and after therapy. At 90 days post-MI, transendocardial injection of either: Combination of CSCs and MSCs (1M/200M, Combo, n=7), MSCs (200M, n=6), or placebo (Plasmalyte, n=6) were administered to the IZ/BZ. Biplane left ventriculography and endocardial border tracings were recorded and compared. TESI was guided to 10 sites in IZ/BZ using ventriculographic wall motion correlated with CMR. Sites were mapped according to the 16-myocardial segment model. We ana...