Suppression of mitogenic activity by stable expression of the regulatory domain of PKCβ

The amino-terminal regulatory domain portion of each protein kinase C (PKC) family member (which in the case of PKCβ1 includes the pseudosubstrate, C1, V1 and C2 domains) plays an important role in regulating the kinase activity of the carboxyl-terminal catalytic domain. To examine the possibility that this regulatory domain region (designated 'PAT') might have biological functions independent of the catalytic domain, we have developed derivatives of R6 cells which stably express a truncated PKCβ1 cDNA that encodes the amino-terminal 317 amino acids, including the entire regulatory domain