Semen energy quick detection reagent kit and its preparation and usage method

The invention discloses a spermatozoon vigor fast detection kit and preparing and using method thereof. The kit includes a spermatozoon collecting box containing a plastic collecting box and spermatozoon liquefacient; triton X100 solution; propidium iodide solution; a standard color card, colors of which are color of adding triton solution with concentration of 0.05-0.2% and propidium iodide solution with concentration of 0.01-0.5% into spermatozoon sample with different concentration notarized by microscope; and a test board containing a shell and a basal plate, wherein two test pores are arranged on center of the shell, absorbent paper and filtering film are equipped on the basal plate. Advantages of the kit are: quantity of activated spermatozoon can be fast and accurately detected, more time can be saved than traditional diagnostic method, and usage is simple.