COMPARISON OF COLONY FORMING UNIT-ASSAY RESULTS OF DIFFERENT HEMATOPOIETIC STEM CELL SOURCES FARKLI HEMATOPOETİK KÖK HÜCRE KAYNAKLARININ KOLONİ OLUŞTURMA COLONY FORMING UNIT-ASSAY KAPASİTELERİNİN KARŞILAŞTIRILMASI

Aim: Hematopoietic stem cell transplantation (HSCT) is practiced successfully especially for hematologic malignancies. The source of hematopoietic stem cells for HSCT is bone marrow in allogeneic transplantation or peripheral blood stem cells after mobilization in autologous transplantation. The aim of this study is to investigate CFU-ASSAY results of two different hematopoietic stem cell sources. Method: Hematopoietic stem cells collected from bone marrow in 7 patients and peripheral blood in 7 patients were included in the study. Hematopoietic stem cells were cultured in semi-solid agar culture medium (Methocult, H 4434 Classic, Stem Cell Technologies, Canada) and CFU-ASSAY was performed after 14-18 day of culture period for each group. Results: CFU-Assay results of peripheral blood stem cells for autologous donors were as follows; CFUGM:28.44x104/kg, BFU-E:40.7x104/kg, CFU-E:0.79x104/kg, CFU-GEMM :1.95x104/kg. CFU-Assay results of bone marrow stem cells for allogeneic donors were as follows; CFU-GM:7.03x104/kg, BFUE:10.46x104/kg, CFU-E:0.16x104/kg, CFU-GEMM:0.51x104/kg. Conclusion: CFU-Assay results revealed that colony forming capacity of peripheral blood stem cells are higher than that of bone marrow stem cells. Peripheral blood stem cells transplantation is becoming more prevalent especially in adults. In pediatric groups, to identify the correlation between invitro results and clinical observations may help to increase preferance of peripheral blood stem cell transplantation.