Study on the stability analysis and radioiodinated prostate cancer specific oncolytic recombinant adenovirus

Objective s 125I is labeled to hTERT/PSA dual regulated proliferation oncolytic gland virus, and then to establish 125I-RSOAds-hTERT/PSA radionuclide oncolytic viral markers, at the same time, to investigate effects of different reaction conditions on the rate of viral markers. Methods N- bromosuccinimide (NBS) markers 125I as the oxidant, the optimal labeling conditions were determined by setting oncolytic virus and NBS with different concentrations respectively. The marker ratios of 125I-RSOAds-hTERT/PSA nuclide oncolytic viral markers in different 125I dosage, reaction time, pH value, reaction volume were observed. Radionuclide oncolytic adenovirus markers were separated and purified by by gel column chromatography. The radioactive purity of 125I-RSOAds-hTERT/PSA markers at different time was determined by paper chromatography. Results The radioactive purity of 125I-RSOAds-hTERT/PSA markers was more than 95%, and it remained stable and remained at 93%~94% for 7 days in the 4 degree refrigerator. In this study, 125 I 0.5 μL (about 0.2 mCi, 7. 4 MBq), NBS 25 μg, 8×109 VP/mL, 125I-RSOAds-hTERT/PSA virus 100 μL, reaction time 3 minutes, PH 7.5, and PBS 120 μL were the optimal reaction condition. Conclusions The method of 125I labeled hTERT/PSA double regulation of proliferation of oncolytic adenovirus is effective and feasible, and the radioactive purity of markers is stable under certain conditions. Key words: Prostatic Neoplasms; Iodine Radioisotopes; Adenoviridae