Presence of two protein kinases in highly purified rat liver nucleoli

Abstract Nucleoli, highly purified as shown by the absence of nucleoplasmic DNA-dependent RNA polymerase, contain two chromatographically separable protein kinases. Nucleoplasm (obtained in the preparation of nucleoli) also contain the same two protein kinases. The proportion of these protein kinases to total protein in these respective fractions indicates that there is no subnuclear localization of either of these enzymes as was demonstrated for the three DNA-dependent RNA polymerases.