T cell expression of the histone demethylase UTX promotes clearance of a persistent virus infection by modulating Tfh cell differentiation (VIR1P.1155)
2015
T cell differentiation and memory formation are impacted by epigenetic changes that modulate nucleosome structure to regulate transcription factor accessibility. The enzymes and specific targets that mediate these processes within T cells are not clear. We show that UTX, a histone H3 lysine 27 (H3K27) demethylase, promotes the formation of the CD4+ T follicular helper cell (Tfh) phenotype during a chronic virus infection. Mice with T cell specific UTX deletion had defects in Tfh cell generation and function that resulted in reduced germinal center responses and virus-specific immunoglobulin production. Furthermore, clearance of the chronic virus infection was specifically dependent on T cell expression of UTX. H3K27me3-ChIP-sequencing and RNA expression analysis revealed a subset of genes that were specifically regulated by UTX via H3K27 demethylation. This group included IL-6Ra, a known regulator of Tfh responses, as well as other genes, which are potentially novel Tfh effector genes. Thus, we identify a critical link between UTX-dependent H3K27 demethylase activity in T cells and immunity to infection.
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