Transgenic Wheat Plants with Trehalose-6-phosphate Synthase (TPS) Gene and Identification of Their Function

It is very important to improve wheat tolerances to drought and salt by genetic engineering for its safe production.A trehalose-6-phosphate synthase(TPS) gene from Saccharomyces cerevisiae related to drought stress and salt tolerance of plant was transferred into CB9945,a common wheat variety,by biolistic bombardment.The T0 putative transgenic plants were tested by PCR,and one plant with TPS was identified.The progenies of T1 and T2 were detected by PCR and liberty detection on leaf,and 15 transgenic lines were obtained finally.Under simulated stress conditions of drought and salt,the transgenic lines were identified for their tolerance to stress,and the result indicated the stress tolerance of the transgenic plants was improved to some extent. The transgenic wheat has been sent to Shanxi province for drought test in field.