Expression and Thrombin Cleavage of Poa p IX Recombinant Allergens Fused to Glutathione S-Transferase

Egil Olsen,Shyam S. Mohapatra

Expression and Thrombin Cleavage of Poa p IX Recombinant Allergens Fused to Glutathione S-Transferase

1992

Egil Olsen
Shyam S. Mohapatra

The high-level expression and purification of Poa p IX recombinant grass pollen allergens were examined utilizing a modified pGEX plasmid, designated as pGEX 2T-1. This vector permi

Keywords:

Gene expression
Affinity chromatography
Plasmid
Expression cloning
Glutathione S-transferase
Fusion protein
Molecular cloning
Biology
Recombinant DNA
Biochemistry
Molecular biology
Thrombin
Enzyme

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