Cloning of MxA Gene from Chickens and Its Expression in Escherichia coli

MxA is a new kind of antivirus protein that is induced by IFN α/β. The MxA gene fragment was amplified by RT-PCR from chicken embryo fibroblast (CEF) cells and subcloned into the pMD-T18 vector and the positive clone was filtrated and the MxA gene was reclaimed and subcloned into the prokaryotic expression plasmid pGEX-6p-1. After recombinant plasmid was induced by Isopropyl β-D-1-thiogalactopyranoside (IPTG), the expressed protein was analyzed by SDS-PAGE, New castle disease virus (NDV) intervence experiment and Vesicular stomatitis virus (VSV)-CEF restrain experiment. Results showed that the sequence of MxA gene amplified by RT-PCR was the same as the sequence in gene map of GenBank and its protein molecular weight was 45 kD, which was the same as the fusion protein GST-MxA. And the recombinant MxA was expressed efficiently in forms of inclusion body with the yield accounting for 30% total bacteria protein by the analysis of the facility.