Latrophilin-like receptor from the parasitic nematode Haemonchus contortus as target for the anthelmintic depsipeptide PF1022A.

SPECIFIC AIMSIn this study, we have tried to identify a possible protein target for PF1022A belonging to a new class of cyclodepsipeptides with broad anthelmintic activity in the parasitic nematode Haemonchus contortus.PRINCIPAL FINDINGS1. The orphan receptor HC110-RUsing PF1022A ligand immunoscreening of a cDNA library constructed from the parasitic nematode H. contortus, we identified a 3539 bp cDNA encoding a novel orphan heptahelical transmembrane 110 kDa receptor, termed HC110-R (Fig. 1A⤻ ). Database and phylogenetic analysis reveal a heptahelical transmembrane protein of 1014 amino acids (B0457.1, protein id: CAA9109.1) of the nematode Caenorhabditis elegans as to be the homolog receptor to HC110-R. Figure 1.The HC110-R protein. A) Alignment of amino acid sequences of H. contortus HC110-R (accession no.: AJ272270) and C. elegans protein B0457.1 (protein id: CAA91091.1). Identical amino acids are marked with asterisks, highly related amino acids with a colon, related amino acids with a single dot. Si...