[Bone marrow stem cell mobilization to the peripheral blood and tumor necrosis factor-alpha expression in ischemic myocardium in rabbit models of hibernating myocardium].

Objective To investigate the changes in peripheral blood bone marrow stem cells and tumor necrosis factor-α gene expression in the ischemic myocardium in rabbit models of hibernating myocardium. Methods Twenty-four male Japanese white rabbits were randomized into 4 groups, including a sham-operated group and 3 model groups with hibernating myocardium induced by partial ligation of the left anterior descending coronary artery. The percentage of CD34-positive cells in the peripheral blood was evaluated by flow cytometry, and TNF-α mRNA expression in the ischemic myocardium was determined by real-time RT-PCR in the 3 model groups (at 3, 7, or 28 days after the operation) and in the sham-operated group. Results In rabbits with partial ligation of the left anterior descending coronary artery, the percentage of CD34-positive cells in the peripheral blood and myocardial TNF-α mRNA expression were significantly increased at 3 and 7 days after the operation in comparison with those in the sham-operated group and those at 28 days postoperatively (P0.01). No significant differences were found in the percentage of CD34 positive cells or myocardial TNF-α mRNA expression between the sham-operated group and the rabbits 28 days after the coronary artery ligation (P0.05). Conclusion Bone marrow stem cell can be mobilized into the peripheral blood in rabbit hibernating myocardium model possibly by increasing TNF-α gene expression in the ischemic myocardium.