Immunity test of bivalent foot-and-mouth disease vaccine using recombinant fowlpox virus as a vector in guinea pigs.

Objective To determine the specific humoral and cellular immunities induced by recombinant fowlpox virus carrying P1-2A-IL18 gene of foot-and-mouth disease virus(FMDV)type Asia-1 and P1-2A-3C gene of FMDV type O in guinea pigs. Methods Guinea pigs were divided into 3 groups and injected i.m.with recombinant fowlpox virus vUTAL-P1-2A-IL18-P1-2A-3C,w-FPV vaccine strain and PBS respectively and determined for splenic T lymphocyte subgroup count,the killing activity of specific CTL and specific antibody against FMDV in sera.Results Both the splenic T lymphocyte subgroup count and the killing activity of specific CTL of guinea pigs immunized with recombinant fowlpox virus vUTAL-P1-2A-IL18-P1-2A-3C were significantly higher than those with w-FPV and PBS.Specific antibodies against FMDV types O and Asia-1 were induced in the guinea pigs immunized with vUTAL-P1-2A-IL18-P1-2A-3C.Conclusion The bivalent FMD vaccine using recombinant fowlpox virus as a vector induced specific humoral and cellular immunities in guinea pigs.