Síntesis de nanopartículas poliméricas de quitosano funcionalizadas con extractos de la mora (Rubrus glaucus) y su evaluación preliminar como agentes antimicrobianos

Synthesis of polymer chitosan nanoparticles funtionalized with blackberryextracts(Rubus glaucus) and its preliminary evaluation as antimicrobial agentsAbstractThe aim of this study is to evaluate the bacteriostatic effect of functionalized chitosan nanoparticles withantioxidants from tropical fruit extracts.In the synthesis and characterization of functionalized chitosan nanoparticles variables are treated in orderto obtain concentrations and other parameters, which shows bacteriostatic effect on some species of pathogenicgram-negative ATCC bacterias: Escherichia coli, Serratia marcescens and Pseudomona aeroginosa, and grampositives:Staphylococcus aureus, Bacillus subtilis and Enterococcus faecalis.The hypothesis assumes that functionalized nanoparticles synthesized according to the ion gelation methodusing tripolyphosphate as crosslinking agent, with modifying agents from blackberry tropical fruit extracts(Rubus glaucus), containing antioxidants, expressed as gallic acid concentration, provides chitosan nanoparticlesthe ability to inhibit bacterial growth.This research performed a preliminary evaluation of the polyphenol content and antimicrobial activity inconjunction with the nanoparticles against pathogens mentioned above. The evaluated fruits exhibit concentrationsof 100 mg of gallic acid/100g and antimicrobial activity against the gram-positive S. aureus E. faecalis andB. subtilis, and the gram-negative S. marcescens determined by disc diffusion method showing an inhibitiondiameter between 10% and 60 % compared to positive control. These results show the potential of thesenanoproducts as antimicrobial agents for use in the pharmaceutical industry.Key words:antimicrobial agents, polyphenols, bacteriostatic effect, chitosan, polimeric