Analysis of the HPLC fingerprint and QAMS from Pyrrosia species

Abstract An effective and comprehensive evaluation method for identifying the origin and assessing the quality of Pyrrosiae Folium has been established, based on analysis of high performance liquid chromatography (HPLC) fingerprint combined with the similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and the quantitative analysis multi-components by single marker (QAMS) method. 20 peaks of the common model were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that 42 Pyrrosia samples were categorized into three groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in the areas of Shanxi, Guangxi and Sichuan provinces. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Pyrrosia herbals by the values of relative correction factors (RCFs) from chlorogenic acid (the internal standard) versus mangiferin, rutin and kaempferol. In conclusion, these methods were successfully applied to identification of the herbs origin and evaluation the quality of Pyrrosia materials. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).