Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class.

Abstract A novel technique for the determination of the concentration of influenza virus antihemagglutinin antibody of the IgG class is described which is based on the following principle: graded doses of purified antibody are allowed to react with homologous virus, resulting in binding of a portion of antibody. The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. The antibody concentrations measured by use of this technique agreed well with chemically determined values.