Identification of Quercetin and Rutin from Propolis Ethanolic Extract (EEP) of Timis County

In this paper were studied the possibilities of some flavonoides (quercetin and rutin) identification from the propolis ethanolic extract (EEP), depending on the harvesting region from the west of Romania (Timis county), the obtained results were compared to those obtained for similar products in literature (Hungary, Czechia, China, Slovenia). Representative samples were collected from three different regions of Timis county (Lugoj 1, Lugoj 2 and Faget). The identification of quercitin and rutin from propolis ethanolic extracts (EEP) was realized with high performance liquid chromatography (HPLC). For separation and quantification the ethanolic extracts of propolis (EEP), I used the high performance liquid chromatography. Quantification was performed on the basis of the calibration curve obtained for pure quercitine. I used the HPLC Hewelett Packard apparatus Agilent 1100 type with UV detection, with the following characteristics: column: Nucleosil C18; 5µm dimension for stationary phase particles; column dimension: 150 x 4,6mm x mm; eluent acetonytrile:water = 1:1, eluent flow: 1 mL/min; wave length: 365 nm; temperature 30°C; injected volume: μL.