Analysis on Concentration of Amino Acids in the Hippocampus of Status Epilepticus Rats with Transplantation of Human Mensenchymal Stem Cells

Objective To observe the concentration changes of amino acids in the hippocampus of status epilepticus(SE)rats with transplantation of human mensenchymal stem cells(hMSC).Methods The SE rat models were induced by Lithium-pilocarpine in DS rats.The SD rats were randomly divided into four groups:hMSC group,phosphate buffered saline(PBS)group,sham group and normal control group.After 10 weeks,all rats hippocampus tissue was taken out and Glu,Asp,GABA and Gly in the hipocampus of the samples were processed and determined by means of high performance liquid chromatography.Results The concentrations of Glu,Asp,GABA in the hippocampus between hMSC group and normal control group were significantly different(P0.05).The concentration of Gly was lower in hMSC group(P=0.0617).The concentrations of Glu,Asp,GABA and Gly in the hippocampus among hMSC group,PBS group and sham group were not significantly different.Conclusions The concentrations of amino acids in the hippocampus of SE rats were lower than that in normal control group.The transplantation of hMSC might not change the concentrations of amino acids obviously in the hippocampus.