Solomon's seal (Polygonatum odoratum) seedlings raised through its seeds can replace the rhizomes impelling higher cost for transplanting, This experiment was done to determine the seed characteristics and the germinating processes to give some information on bulk production of seedlings using the seeds. The external or internal morphology of seeds or seedlings grown in lab. or greenhouse was examined mainly with stereomicroscope. The external shape of Solomon's seal seed was hard seed-coat and orthotropous ovule with linear type embryo stretching to the center of seed. Germination proceeded through the several steps. The lower part of seed embryo having the primordia of bulbil and roots first grew before the bulbil and roots was developed from the primordia. The lower part of embryo was enlarged toward the endosperm of seed as soon as seed germinated. Then epicotyl was formed on the apex of bulbil. The epicotyl was elongated after at least 6-week chilling treatment for breaking its dormancy and the first leaf shape was affected by light intensity given during seedling emergence. The bulbil was the first organ of the rhizome used as tea or herb medicine.
Campanulaceae having the most growing areas among medicinal crops cultivated in Korea occasionally failed to establish a reasonable standing in practice. The experiment was carried out to examine the effect of prechilling (0 : 4 : 8 days), light quality (red : white : dark) and daily irradiation hours (8 : 12 : 16) after sowing on seed germination and radicle elongation of Campanulaceae (Platycodon grandiflorum : Codonopsis lanceolata : C. pjlosula) to give an information on their earlier standing establishment. Mean germination rate of P. grandiflorum was the highest but that of C. pilosula was the lowest regardless of all the treatments. 12 hours irradiation or prechilling increased to 8 days enhanced their earlier or later germination, respectively. White light increased the rate of P. grandiflorum but alleviated that of C. lanceolata regardless of the daily irradiation hours. Although prechilling eliminated such effect of white light, light quality treatment effect on their mean germination rates was influenced by period after sowing, daily irradiation hours or prechilling. On the 9th day after sowing, C. lanceolata showed the greatest radicle length, and both daily 8 hours irradiation and 8 days prechilling enforced to elongate their radicles, while P. grandjflorum and C. lanceolata more lengthened their radicles in all prechilling treatments than in no chilling but C. pilosula showed the similar result only in the 8 days prechilling.
Flavonoids biosynthesis pathway mostly determines the flower colour pheno-types in plants. This study was carried out to determine the presence and expression rates of the flavonoids biosynthesis-related genes in major lily cultivars. Primers of major colour genes were prepared by comparing the homologous genes of other species like sorghum, onion, and rice, and used for RT- PCR of eight lily cultivars. The results of transcriptional rate analyses showed that, in pink-flowering lily cultivars like, 'Pirax', red-flowering cultivar 'Atika', yellow-flowering cultivar 'Gironde', and white cultivar 'Siberia', all the genes tested were expressed. However, F3'H and DFR genes were not expressed in yellow-flowering cultivar 'Yelloween'. In white-flowering cultivars 'Samarinde' 'Richmond' and 'White Elegance', gene expressions varied among cultivars, especially in the genes CHS, F3H and ANS. We assume that the results on the presence and/or expression rates of the major flavonoid-biosynthesis genes shall be useful in the hybridization and transformation breeding in lilies for flower colour modification.
Orostachys japonicus, a monocarpic plant, is artificially grown in greenhouses. The study was carried out to examine the effect of shading (0, 35, 55, 75, 95%) on growth, morphological characters, and flowering. The treatments were done on August 25 and afterward samples were taken every 2 weeks until October 20, in which growth, morphological and flowering related characters were measured. With severer shading plant height, inflorescence length, number of leaves including bracts and stem diameter were decreased although severely declined in 95% shading treatment. Leaves and bracts, stem, root, shoot and total dry weights increasingly declined with severer shading. Florets formed on the inflorescence showed similar response to the shading treatment as plant height did. Flowering of the florets was not observed throughout all the sampling period, meaning that different light intensities by shading did not affect their flowering.
In bean sprout culture water imbibition and benzyladenopurine (BA) treatment are done at the same time. The study was carried out to check the effect of treatment period (3, 5, and 7 hours) on growth of mungbean (cv. Keumseongnogdu, Owoolnogdu, and Zhong Lu 1) sprouts and to analyse its absorption amount on the base of their moisture content. The 3 cultivar seeds were soaked in 50 ppm BA solution immediately before 3 hour aeration and then cultured for 6 days. The sprouts were sorted by 4 categories on the base of hypocotyl length; > 7cm, 4 to 7cm
Benzyladenopurine (BA), a synthetic chemical commonly used for bean sprout culture, should be minimized for wellbeing foods or not be applied. The study was done to check the effect of different BA concentrations treated during 5 hour imbibition on growth and morphological characters of mungbean sprouts. The mungbean seeds of 3 cultivars (Keumseongnogdu, Owoolnogdu, and Zhong Lu 1) were imbibed for 5 hours in the solutions with different BA concentrations (0, 25, 50, 75, and 100 ppm) before 3 hour aeration. On the 6th day after culture, the mungbean sprouts were classified by 4 categories on the base of hypocotyl length; > 7cm, 4 to 7cm,
Lateral roots formed on mungbean sprouts should lower their quality. The study was carried out to clarify the effects of aeration periods (AP; 1, 2, 3, and 4 hours) and temperature (AT; 20, 30, and ) after 5 hour seed imbibition into 50 ppm benzyladenopurine (BA) solution on growth and morphological characters of mungbean (cv. Keumseongnogdu, Owoolnogdu and Zhong Lu 1) sprouts. On the 6th day, the mungbean sprouts were sorted by 4 categories on the base of hypocotyl lengths; > 7cm, 4 to 7cm,
Several presowing seed treatments have been used to facilitate the seed germination and seedling emergence. The study was performed to design a model of presowing seed treatments in Taraxacum platycarpum. Germination and seedling growth responses to temperature, drying, chilling, and priming were investigated to know the optimum germination conditions and presowing treatment. The optimum temperature condition for germination and seedling growth were at dark condition. The seeds exposed to infra red light, however, did not germinate. The moisture content of seeds dried at for 3.5 hours was returned to the level of unimbibed seeds. The germination of seeds dried under dark condition was better than those dried under infra red light. Prechilling treatment imposed for 6 weeks showed higher germination rate than treatment or priming. In field test, the highest field emergence was observed in the seeds dried after prechilled for 6 weeks and followed by drying followed by > low temperature > priming. Imbibition with 0.1 mM solution for 2 hours then drying at 35 for 3.5 hour under dark condition was effective to the practical presowing treatment for Taraxacum seeds.
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