The present study was performed in order to investigate the mechanism(s) involved in eotaxin-induced normal human eosinophil chemotaxis using a 48-well micro-chemotaxis chamber assay. Eotaxin, at a wide range of doses, induced eosinophil chemotaxis with optimal activity at 100 ng/mL. To elucidate the role of Ca2 + as a second messenger, eosinophils were depleted of intracellular Ca2 + which, per se, did not modify eosinophil chemotaxis. To gain insight of the possible intracellular signal transduction, we blocked pertussis toxin (PTX)-sensitive Gj proteins as well as several protein kinases. It was found that the inhibition of tyrosine kinase with herbimycin A and the inhibition of mitogen-activated protein kinase (MAPK) with MEK-1 inhibitor (PD98059) significantly blocked chemotaxis; however, inhibition of protein kinase C with staurosporine, protein kinase A with H-89 and Gi proteins with PTX did not affect chemotaxis. These results suggest a signal transduction pathway(s) involving Ca2 +-independent tyrosine kinase and MAPK activities.

Chemotaxis assay

Staurosporine

10.2332/allergolint.47.9

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Citations (5)

Absorption of light in evaporated cadmium telluride thin films in the region of the fundamental absorption edge

Thin Solid Films (1981)

A. A. El-Shazly H. T. El-Shair M. K. El-Mously

Cadmium telluride photovoltaics

Absorption edge

Photon energy

Absorptance

Two-Photon Absorption

10.1016/0040-6090(89)90596-8

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Citations (12)

Impact of cold storage and blending different lactations of cow's milk on the quality of Domiati cheese

African Journal of Food Science (2010)

Magdy Mohamed Ismail El-Tahra Ammar A. A. El-Shazly M. Z. Eid

The present investigation studied the making of Domiati cheese from raw or pasteurized cow’s milk stored at 4 - 5°C for 24 or 48 h as well as the effect of mixing equal amounts of evening and morning milk with cold stored cow's milk on some properties of Domiati cheese. Domiati cheese made from cooled stored cow's milk, either raw or pasteurized, had lower yields, TS, fat, TN and higher acidity, salt, salt in moisture, WSN, WSN/TN, NPN, NPN/TN and TVFA contents relative to the control. Adding of evening and morning milks to raw or pasteurized refrigerated stored milk decreased the yield, acidity, WSN, WSN/TN, NPN, NPN/TN and TVFA and raised TS, fat, salt, salt in moisture, TN and TN/DM values of cheese. Mixing of raw or pasteurized evening and morning milk with cold stored milk had no clear effect on free amino acids (FAA) contents of cheese. Cooling of cow's milk for 24 or 48 h decreased concentrations of the majority of FAA of Domiati cheese stored for 90 days. Adding evening and morning milks to cold stored milk or preservation of both raw and pasteurized cow's milk for 24 or 48 h at 4 - 5°C raised the numbers of various microbial groups. Key words: Milk preservation - morning and evening milks- soft cheese.

Pasteurization

Farmer cheese

Cow milk

Milking

10.5897/ajfs.9000160

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Citations (22)

Optical and Electrical Properties of CdS Thin Evaporated Films

Journal of Optics (1981)

A. A. El-Shazly M.M. El-Nahass M. A. Kbnawy Taghreed Hussien

Vacuum evaporation

10.1007/bf03549037

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Citations (1)

Actin reorganization is involved in vasoactive intestinal peptide induced human mast cells priming to fraktalkine-induced chemotaxis

International Journal of General Medicine (2008)

A. A. El-Shazly

We recently reported a novel neuro-immuno co-operation between vasoactive intestinal peptide (VIP) and fraktalkine (FKN) in recruiting human mast cells to the asthmatic airway that provided a classical example of priming effect on mast cells migratory function, but the role of the F-actin in human mast cell chemotaxis' priming is poorly defi ned.Therefore the aim of this study was to further investigate the biophysical role of the cytoskeletal element; the F-actin, intracellular reorganization and its polymerization in mast cell priming of chemotaxis function.In the present communication it is shown by immunofl uoresence confocal microscopy analysis that physical F-actin intracellular reorganization in a membrane bound manner on human mast cell is involved in VIP-induced priming of human mast cell chemotaxis against FKN.The F-actin reorganization was calcium independent and without modifi cation of its contents as assessed by fl uorescence-activated cell scanning analysis.These results identify a novel role for the biophysical association of F-actin in the crosstalk between neuro-infl ammatory mediators and mast cells and may be an important target for therapeutic modalities in allergic infl ammation.

Crosstalk

Priming (agriculture)

10.2147/ijgm.s3759

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Optical properties of Cd 3 As 2 polycrystalline thin films

Vacuum (1996)

A. A. El-Shazly H. S. Soliman H.E.A. El-Sayed Da Abd El-Hady Da Abd El-Hady

Source

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Citations (0)