The waxy gene (Wx) in rice, which encodes the granule bound starch synthase enzyme, is responsible for amylose synthesis. Glutinous (sticky) rice has little or no amylose that can be used in various applications, such as brewing. In this study, knockout of the Wx gene with CRISPR/Cas9 technology was conducted in two elite japonica rice lines, Huaidao 5 (HD5) and Suken 118 (SK118), aiming to develop elite sticky rice varieties. We achieved six homozygous T0 plants with more than 200 bp deletion in the Wx gene, as well as 36 wx-HD5 and 18 wx-SK118 homozygous transgene-free plants in the T1 generation. The seeds of all the mutants were white and opaque, similar to those of sticky rice, and contained only 2.6%–3.2% amylose. Results of scanning electron microscopy showed that the quality of rice did not change. In conclusion, we successfully developed two elite sticky rice varieties.
XL12,YN3,N2,H14 were selected for repressing the pathogen of tobacco brown spot disease.After fermenting for 24h,48h,72h,96h and taking those fermentation fluid treatmented as 200×,400× to inhibit the sprout rate of spores after 12h.The results indicated that XL12 was superior to others and fermented for 48h has the obvious inhibition.
According to the problem of long time waiting for registration,paying and inspection and short time for diagnosis and treatment in hospital which revealed in the traditional outpatient service process and based on the existing medical information system in a hospital,with the conception of integration,a self service medical system fitting for hospital has been designed and implemented.A series of self service functions such as starting card,registration,report fetching and payment have been designed which has achieved data seamless transformation between HIS,LIS,Unionpay,Medical insurance and Citizen card,so the patient' s medical information has integrated and has provided basic information for building electronic health document.
In order to improve the precision of marker-assisted selection for S5-n,a functional marker S5136 based on the 136 bp deletion in the DNA sequence of S5-n was developed.The sterile line Yuetai A with S5-n has been identified with marker S5136,and conformed by sequencing.There was a SNP in the downstream of the 136 bp DNA deletion region of Yuetai A,which has been discovered in the wide compatible germplasm resources from India.The polymorphism was identified with marker S5136 between the photo-thermo sensitive sterile line Peiai 64S and the parent R986.Therefore S5136 was a perfect marker to test the purity of commercial hybrid seed of Liangyou 986(Peiai64s/R986).Four Peiai 64S plants were identified from the 233 sampled plants with S5136,with self seed setting rate of Peiai64S being 1.72%.The PCR efficiency was compared between the SDS DNA extracting method and the simple-way DNA extracting method.The results showed that the DNA extracted by the simple-way was better,except that the DNA could not be kept for more than 2 weeks at room temperature.Therefore,the functional marker S5136 was a powerful molecular marker in S5-n germplasm screening and seed purity testing for the two line hybrids with Peiai 64S as maternal parent.
Dendrobium nobile Lindl.(Chinese name Jin-Chai-Shi-Hu) is a species of Orchidaceae with distribution in Southeast Asia,southwest and south of China.In this work,a water-soluble heteropolysaccharide DNP-W1B with a molecular mass of 7.7×105 and specific rotation of [α]20D=+81.3°(c 0.3,H2O) was isolated from the stems of D.nobile Lindl.Monosaccharide composition analysis showed that DNP-W1B contained glucose,arabinose and galactose in a molar ratio of 6.2: 3.1: 0.9.The structural features of DNP-W1B were revealed by the combination of chemical and instrumental analysis,including IR,GC,methylation analysis and NMR.The results showed that DNP-W1B possessed a backbone of a disaccharide unit of [→4)-β-Glcp-(1→6)-β-Glcp-(1→] with 33% branches at O-4 of(1→6)-linked β-D-glucopyranosyl residues.The side chains contained arabinosyl and galactosyl chains.Primary immunological tests in vitro indicated that it could stimulate LPS-induced B lymphocyte and Con A-induced T lymphocyte proliferation.Survey showed that DNP-W1B was a novel immunoactive polysaccharide compound from Dendrobium Sw.
Thunnus is a important commercially marine fish species,which is widely distributed.Thunnus has been intensively studied in China,including its basic biological characteristics in fishery and artificial reproduction.However,very few studies on Thunnus were focused on genetic diversity,which has become an obstacle to further researches on the protection,management,rational exploitation and sustainable utilization of Thunnus resources.The genetic diversity of wild Pacific blue-fin tuna(Thunnus thynnus)and Pacific yellow-fin tuna(Thunnus albacare)were investigated by using amplified fragment length polymorphic(AFLP) in the present paper.AFLP fingerprinting was regarded as one of the most useful techniques in nowadays for DNA fingerprint analysis.The results showed that a total of 675 DNA amplification bands ranging from 100~750 bp were produced using 9 pairs of primer combinations,and 35~54 polymorphic loci were detected per primer combination.The percentage of polymorphic loci and Shannon's information index of the blue-fin and yellow-fin tuna were 57.48%,0.330 1 and 54.52%,0.301 8,respectively.Both populations were at the same high level of genetic diversity.AMOVA analysis(83.79%) indicated that a certain extent of differentiation in two species.There were 83.79% of variance was among the populations and 16.21% of variance was within a population.Compared with other fishes,both tuna species possessed rich population genetic diversities.The information of genetic diversities in different populations will give us theoretical guidance in breeding and genetic improvement.
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