Abstract Two novel benzothiadiazole derivatives were prepared. Clear color changes from orange‐red to blue (DPCN) and pink to green (DPTPE) were observed after the addition of fluoride ions (F − ). DPCN and DPTPE displayed ratiometric fluorescence changes in the presence of F − . 1 H‐NMR titration and DFT calculations confirmed deprotonation in the presence of F − . Compared with DPTPE, the hydrogen proton of the imine group in DPCN had higher activity due to the influence of the strong electron‐withdrawing cyano group, such that it was more easily deprotonated and the detection limit for F − was much lower. This work provided guidance for the further design and preparation benzothiadiazole based colorimetric and ratiometric fluorescence sensor for F − .
A novel Escherichia coli-Mycobacterium shuttle vector pMSL was constructed for the purpose of utilizing Mycobacterium smegmatis to express and purify Mycobacterium tuberculosis proteins.The vector contains the following: the promoter region of the hsp60 gene from M.bovis,a cloning site for inserting the target genes,an enhanced green fluorescence protein(EGFP) for monitoring the expression and the localization of target proteins,a thrombin cleavage site integrated between cloning site and EGFP gene for the removal of EGFP from purified fusion proteins,and the codons encoding histidine tag(His6) fused at the end of EGFP gene.The results indicated that the M.smegmatis transformed with pMSL could express EGFP efficiently.The EGFP protein thus expressed was purified effectively using the nickel resins via affinity chromatography.
The scarcity of Li along with its uneven distribution in earth has become a great challenge in the field of electrochemical energy storage. Replacing Li with Na may address the above issues, as Na and Li are very similar in electrochemical characteristics, and Na is abundant in nature with low cost. Herein, the Na-ion conducting gel polymer membranes comprising a 2-HEC/EMITf/NaTf system are synthesized by employing the solution-casting method. The effects of NaTf salt and EMITf ionic liquid on the electrical and electrochemical properties of membranes are investigated. It is demonstrated that the salt is dissolved in the polymer matrix, thereby raising its ionic conductivity (IC), which is further raised by ionic liquid incorporation. The optimized membrane (2-HEC:EMITf:NaTf = 1:0.6:0.15 in mass, named as GPE/Na-3) exhibits a high room-temperature IC of 1.11× 10–3 S cm–1 along with a wide electrochemical stability window of 4.8 V. The membrane also displays sound tensile strength and breaking strain (4.5 MPa and 94.4%, respectively). As the electrolyte, the optimized membrane is combined with graphene electrodes to fabricate a supercapacitor. The supercapacitor presents a high capacitive behavior with an appealing cyclic stability. As evident from the above performance, it is believed that the Na-ion conducting gel polymer membrane possesses potential applications in future Na-ion energy storage devices.
Muscle development requires myoblast differentiation and muscle fiber formation. Myod family inhibitor (Mdfi) inhibits myogenic regulatory factors in NIH3T3 cells, but how Mdfi regulates myoblast myogenic development is still unclear. In the present study, we constructed an Mdfi-overexpression (Mdfi-OE) C2C12 cell line by the CRISPR/Cas9 system and performed RNA-seq on Mdfi-OE and wild-type (WT) C2C12 cells. The RNA-seq results showed that the calcium signaling pathway was the most significant. We also established the regulatory networks of Mdfi-OE on C2C12 cell differentiation and muscle fiber type transformation and identified hub genes. Further, both RNA-seq and experimental verification demonstrated that Mdfi promoted C2C12 cell differentiation by upregulating the expression of Myod, Myog, and Myosin. We also found that the positive regulation of Mdfi on fast-to-slow-twitch muscle fiber transformation is mediated by Myod , Camk2b , and its downstream genes, such as Pgc1a , Pdk4 , Cs , Cox4 , Acadm , Acox1 , Cycs , and Atp5a1 . In conclusion, our results demonstrated that Mdfi promotes C2C12 cell differentiation and positively modulates fast-to-slow-twitch muscle fiber transformation. These findings further our understanding of the regulatory mechanisms of Mdfi in myogenic development and muscle fiber type transformation. Our results suggest potential therapeutic targets for muscle- and metabolic-related diseases.
Abstract Trace detection of nitroaromatic explosives in aqueous solution is important for environmental pollution evaluation and terrorist prevention. Here, a new pyrenyl‐based aggregation‐induced emission (AIE) fluorochrome (CPPyV) with desirable fluorescent performance was synthesized and applied to detect 2,4,6‐trinitrotoluene (TNT) in water. The fluorescence of CPPyV can be selectivity and efficiently quenched by TNT. The Stern‐Volmer constant (K SV ) of CPPyV for TNT was determined to be 6.2×10 5 M −1 , with a low detection limit of 4 nM. Notably, CPPyV could be used for TNT determinations in real water samples and also developed as a fluorescent paper sensor which could efficiently detect TNT by naked‐eye when the TNT concentration was 1.0×10 −8 M. The quenching process was dynamic quenching mechanism and the theoretical calculations revealed that photoin‐duced electron transfer (PET) from excited CPPyV to electron deficient TNT was the main reason for fluorescence quenching. The excellent detection performance of CPPyV yielded it a promising sensor material for actual field applications.
ObjectiveThe aim of this study was to evaluate safety and efficacy of thoracic endovascular aortic repair (TEVAR) for acute Stanford type B aortic dissection (TBAD) with retrograde type A intramural hematoma (TAIMH).MethodsPatients with acute TBAD with retrograde TAIMH treated with TEVAR between January 1, 2014, to March 31, 2022, were retrospectively reviewed. Aortic diameter and distance were measured using the 3D Slicer image computing platform. Patients' characteristics, procedural, in-hospital and follow-up data, and aortic remodeling were analyzed.ResultsFifty-two patients (average age, 52.6 years; 42 males [80.8%]) were included. The median interval from symptom onset to TEVAR was 11 days (interquartile range, 7.0-16.8 days). The maximal diameter of the ascending aorta (AA) was <50 mm, and the hematoma thickness in the AA was ≤10 mm in all patients. Both the in-hospital and 30-day mortality rates were 0%. The 30-day complication rate was 11.5%. The overall cumulative survival rates were 100% at 1 year, 97.1% at 3 years, and 92.6% at 5 years. Four of 52 patients (7.7%) developed retrograde type A aortic dissection at 10 days to 4 months postoperatively, and one of 52 patients (1.9%) developed an isolated AA dissection 4 months postoperatively; these five patients were treated and alive at late follow-up in March 2022. The rates of cumulative freedom from thoracic aortic re-intervention were 93.7% at 1 year and 90.7% at 5 years. Positive AA remodeling was observed in 92.3% (48/52) of patients during follow-up. The maximal diameter of AA (mean ± standard error of mean) at admission was 42.7 ± 0.8 mm, which decreased to 39.5 ± 0.9 mm at last follow-up. The maximal AA hematoma thickness at admission was 7.6 ± 0.3 mm, which reduced to 2.2 ± 0.9 mm at last follow-up.ConclusionsFor selected patients of acute Stanford TBAD with retrograde TAIMH, endovascular repair may be a safe, effective, and durable alternative treatment, if the maximum diameter of the AA is <50 mm and the intramural hematoma thickness in the AA is ≤10 mm.
High-quality conjugated microporous polymer (CMP) films with orientation and controlled structure are extremely desired for applications. Here, we report the effective construction of CMP 3D composite films (pZn/PTPCz) with a controlled porosity structure and preferred orientation using the template-assisted electropolymerization (EP) approach for the first time. The structure of pZn/PTPCz composite thin films and nitrophenol sensing performance were thoroughly studied. When compared to the control CMP film made on flat indium tin oxide (ITO) substrates, the as-prepared pZn/PTPCz composite films showed significantly enhanced fluorescent intensity and much better sensing performance for the model explosive. This was attributed to the metal-enhanced fluorescence (MEF) of porous nanostructured zinc (pZn) and the additional macroporosity of the pZn/PTPCz composite films. This work provides a feasible approach for creating oriented 3D CMP-based thin films for advanced applications.
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