A broad forensic screening method for 256 analytes in whole blood based on a fully automated SPE robotic extraction and ultra-high-performance liquid chromatography (UHPLC) with TOF-MS with data-independent acquisition has been developed. The limit of identification was evaluated for all 256 compounds and 95 of these compounds were validated with regard to matrix effects, extraction recovery, and process efficiency. The limit of identification ranged from 0.001 to 0.1 mg/kg, and the process efficiency exceeded 50% for 73 of the 95 analytes. As an example of application, 1335 forensic traffic cases were analyzed with the presented screening method. Of these, 992 cases (74%) were positive for one or more traffic-relevant drugs above the Danish legal limits. Commonly abused drugs such as amphetamine, cocaine, and frequent types of benzodiazepines were the major findings. Nineteen less frequently encountered drugs were detected e.g. buprenorphine, butylone, cathine, fentanyl, lysergic acid diethylamide, m-chlorophenylpiperazine, 3,4-methylenedioxypyrovalerone, mephedrone, 4-methylamphetamine, p-fluoroamphetamine, and p-methoxy-N-methylamphetamine. In conclusion, using UHPLC-TOF-MS screening with data-independent acquisition resulted in the detection of common drugs of abuse as well as new designer drugs and more rarely occurring drugs. Thus, TOF-MS screening of blood samples constitutes a practical way for screening traffic cases, with the exception of δ-9-tetrahydrocannabinol, which should be handled in a separate method.
The goal of this investigation was to determine the pattern of fatal poisonings in eastern Denmark from 1998 to 2002 and compare it with similar investigations from 1979 to 1996.The material included 2,996 autopsies from eastern Denmark in which extensive forensic chemical investigations were performed.Of the 2,996 autopsies, 694 cases were drug addicts, in whom 497 fatal overdoses were detected, while in the remaining 2,302 cases of nonaddicts, 443 fatal poisonings were determined. Morphine (heroine) and methadone were the main causes of death among the fatal poisonings of the drug addicts, accounting for 90% of the cases. The fatal poisonings among the nonaddicts were due mainly to medicine (73% of the cases); 13% were due to carbon monoxide and/or cyanide poisoning, and 12% were due to acute ethanol poisoning. The medicine was a wide range of strong and weak analgesics, antidepressants and antipsychotic drugs of older and newer origins. Comparison with earlier investigations from 1979 to 1996 showed that the poisoning pattern had changed, but similarities were also seen. The most frequently occurring drugs involved in fatal poisonings in eastern Denmark from 1979 to 2002 were morphine, methadone, ethanol and carbon monoxide/cyanide, of which methadone increased in occurrence over the 23-year period.The investigation of fatal poisonings is an important element in monitoring changes in drug abuse and poisoning patterns and levels.
Purpose – This paper aims to outline the benefits of using resilience assessment instead of command and control mechanisms to evaluate sustainable campus environments. Design/methodology/approach – An exploratory mixed-method design was followed for the purposes of the project. During the first qualitative phase, a historical timeline of the focal system was created. In the quantitative phase, the resilience assessment guided the investigation. To collect data, the case study research strategy included a heuristic process of collecting and reviewing documents, semi-structured interviews, observations and the systematic implementation of the resilience assessment approach. Findings – Based on the resilience assessment approach, it is argued that the environmental status of university campuses can be considered relevant to the local community and immediate environment. Knowledge of the finite resources and their capacity in the context of the social-ecological system may increase the resilience of a campus. Originality/value – This research study explores the use of an alternative approach to environmental practices at university campuses. The resilience assessment is usually performed on large ecosystems. By applying this approach to a small ecosystem, the study fills a gap in the applicability of the resilience approach.
In this investigation about 900 saliva samples, collected from drivers not suspected to be under the influence of drugs or alcohol were screened by Cozarts Drugs of Abuse Microplate EIA for benzodiazepines, amphetamine, cannabis, cocaine/benzoylecgonine and opiates. The screening only includes few of the benzodiazepines on the market in Denmark, which means, that only a part of the saliva samples positive for benzodiazepines will be detected by the screening, and that the number of positive benzodiazepine samples will be underestimated. All the saliva samples positive for drugs by the screening were analysed by specific methods, mostly gaschromatography-mass spectrometry (GC-MS). Totally 2% of the investigated saliva samples were positive for drugs. 0.7% (6) of the investigated saliva samples were positive for benzodiazepines, 0.8% (7) for cannabis (tetrahydrocannabinol-THC), 0.3% (3) for opiates, 0.1 % (1) for amphetamine and 0.1% (1) for cocaine/benzoylecgonine. An evaluation of the importance of the saliva concentration will be described. (A) For the covering abstract of the conference, see ITRD Abstract No. E201067.
A considerable number of cancer patients use complementary medicine therapies in order to alleviate different symptoms such as pain, anxiety, and depression, occurring in connection with cancer. This paper explores the question to what extent massage therapies are able to reduce the amount of pain, anxiety, and depression. For this purpose, a systematic literature analysis was carried out in the electronic databases and specialist journals. There is already evidence that massage therapies can influence the symptoms of pain, anxiety, and depression in a positive way.
Abstract We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with a description of a newly developed HRAM-LC-MS method used for identification. As examples of identification, we present data for the peptides AOD-9604, [D-Ala 2 , Gln 8 , Ala 15 , Leu 27 ]sermorelin and the protein follistatin. It was found that a method with minimum sample preparation could be implemented for all of the confiscated peptides and the protein somatropin. However, for the protein follistatin it was necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time.
An isocratic reversed-phase high-performance liquid chromatography (HPLC) method is developed and validated for the quantitation of 3,4-methylenedioxymethamphetamine (MDMA) in tablets. The chromatographic separation is achieved with potassium phosphate buffer (pH 3.2)-acetonitrile (9:1, v/v) as mobile phase, a Chromspher B column, and UV detection at 210 nm. The calibration curve is linear from 1.4 to 111 µg/mL. The percent relative standard deviation for intra- and interday precision studies is 2.7% each. The measurement uncertainty is estimated to 9%. The method is specific and successfully used for routine quantitation of MDMA in tablets.
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