Silvia Nascimento

Universidade de São Paulo

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Raquel Salomone

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Ricardo Ferreira Bento

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Heloisa Juliana Zabeu Rossi Costa

Universidade de São Paulo

Karina Lezirovitz

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Alfredo Luiz Jácomo

Universidade de São Paulo

Flávio Hojaij

Universidade de São Paulo

Heloísa Albuquerque Costa

Universidade de São Paulo

J. Aguiar

Anísio Neto de Oliveira Castelo Branco

Centro Universitário UNINOVAFAPI

Ana Almeida

Polytechnic Institute of Porto

Cooperative Institutions

Universidade de São Paulo

Centro Universitário UNINOVAFAPI

Hospital Universitário da Universidade de São Paulo

Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

Universidade Brasil

Centro Universitário das Faculdades Metropolitanas Unidas

Polytechnic Institute of Porto

University of Coimbra

Santa Casa Hospital

Faculdade de Ciências Médicas da Santa Casa de São Paulo

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Polyethylene glycol fusion associated with antioxidants: A new promise in the treatment of traumatic facial paralysis

Head & Neck (2018)

Raquel Salomone Alfredo Luiz Jácomo Silvia Nascimento Karina Lezirovitz Flávio Hojaij

Abstract Background Recent studies in invertebrates have taught us that early cell membrane regeneration is determinant for axonal recovery and survival after trauma. Many authors obtained extraordinary results in neural regeneration using polyethylene glycol fusion protocols, which also involved microsutures and antioxidants. Methods Sixty rats were evaluated with functional and histological protocol after facial nerve neurotmesis. Groups A and B had their stumps coapted with microsuture after 24 hours of neurotmesis and groups C and D after 72 hours. In addition to the microstructure, groups B and D used the polyethylene glycol‐fusion protocol for the modulation of the Ca +2 . Results At the sixth week, the latency of group D and duration of group B was lower than groups A and C ( P = .011). The axonal diameter of the groups that used polyethylene glycol‐fusion was higher than those who did not use polyethylene glycol‐fusion ( P ≤ .001). Conclusion Although not providing a functional improvement, polyethylene glycol‐fusion slowed down demyelination.

10.1002/hed.25122

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COMPARATIVE ANALYSIS OF THE EVOKED POTENTIAL AUDITORY OF THE BRAINSTEM WITH THE MAGNETIC NUCLEAR RESONANCE IN THE DIAGNOSIS OF NEURINOME OF VIII THE CRANIAL PAIR

International Archives of Otorhinolaryngology (2012)

Silvia Nascimento Carlos A. V. Rodrigues Dennyson Almeida

10.7162/s1809-9777201200s1o-009

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Mandibular Branch of the Facial Nerve in Wistar Rats: New Experimental Model to Assess Facial Nerve Regeneration

International Archives of Otorhinolaryngology (2014)

Ricardo Ferreira Bento Raquel Salomone Ricardo de Souza Alves Ferreira Ciro Silva Heloísa Albuquerque Costa

Introduction The ideal animal model for nerve regeneration studies is the object of controversy, because all models described by the literature have advantages and disadvantages. Objective To describe the histologic and functional patterns of the mandibular branch of the facial nerve of Wistar rats to create a new experimental model of facial nerve regeneration. Methods Forty-two male rats were submitted to a nerve conduction test of the mandibular branch to obtain the compound muscle action potential. Twelve of these rats had the mandibular branch surgically removed and submitted to histologic analysis (number, partial density, and axonal diameter) of the proximal and distal segments. Results There was no statistically significant difference in the functional and histologic variables studied. Conclusion These new histologic and functional standards of the mandibular branch of the facial nerve of rats establish an objective, easy, and greatly reproducible model for future facial nerve regeneration studies.

10.1055/s-0034-1366977

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Lacrimal sac mucocele

Brazilian Journal of Otorhinolaryngology (2014)

Silvia Nascimento Ana Baleska Rodrigues Talline Priscila Magalhães Jurity Jéssica Coelho de Sá Anísio Neto de Oliveira Castelo Branco

Paciente do sexo feminino, 54 anos, apresentava queixa de lacrimejamento excessivo por olho direito ha 12 anos. Apos quatro anos, apresentou obstrucao progressiva das fossas nasais. Ha um ano surgiu abaulamento proximo ao canto interno do olho direito, doloroso a compressao. Ao exame fisico, havia epifora em olho direito e lesao de consistencia cistica, com hiperemia da pele sobrejacente proxima ao can-to medial do olho direito, de 1,2 cm de diâmetro (fig. 1A). A lesao nao apresentava drenagem a expressao, tampouco regurgitacao de secrecao pelos canaliculos. A rinoscopia anterior, foi visualizada obstrucao total da fossa nasal direi-ta e, a esquerda, reducao de sua luz por desvio septal. Tomografia mostrou lesao com consistencia de partes moles na localizacao do etmoide, seio maxilar e fossa nasal direi-tos, determinando erosao da lâmina papiracea com com-pressao do reto medial e deslocamento do globo ocular, alem de erosao e deslocamento do septo nasal para a esquerda, compativel com mucocele (fig. 1B). Sendo assim, optou-se por tratamento cirurgico, que revelou bolsa pre-enchida por material mucopurulento e originaria do saco

Mucocele

10.1016/j.bjorl.2014.05.001

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Polyethylene Glycol fusion associated with anti‐oxidants: A new promise in the treatment of traumatic paralysis

Head & Neck (2018)

Raquel Salomone Alfredo Luiz Jácomo Ricardo Ferreira Bento Silvia Nascimento Karina Lezirovitz

Contrary to what is described in our article1, Bittner et al.2 and Bamba et al.3 evaluated the composite action potential (CAP), instead of composite muscular action potential (CMAP). The correct legend of Figure 1 is: Figure showing the steps of the PEG-fusion experience: A- Surgical preparation of stumps following by bathing the stumps in a hypotonic Ca2 -free Krebs physiological saline and bath the stumps with methylene blue (MB) in hypotonic Ca2+-free saline for 3 minutes. B- Microsuture. C- Bathing the nerve in a hypotonic Ca+2 free PEG solution (double distilled H2O) for 2 minutes D- Bathing the nerve in isotonic Ca+2 containing (Krebs) for 3 minutes. The method was not fully described in the article, so for a better accuracy and reproducibility, we supplement the information with the following: PEG-fusion was carried out bathing the stumps in hypotonic Ca + 2 free Krebs physiological saline (0,5 mM EGTA, 99 mM NaCl, 5 mM KCl, 1,2 Mm KH2PO4, 1,3 mM MgSO4, 26 mM NaHCO3, 10 mM sodium ascorbate, 10 mM dextrose, pH 7,35 in 319 mOsm double distilled H2O); 100 μM methylene blue in hypotonic Ca2 + −free saline for 3 minutes (Figure 1A), prior to the sutures. After sutures (Figure 1B), the resulting nerve was bathed in a 500 mM PEG solution dissolved in double distilled H2O for two minutes (Figure 1C). Last, isotonic Ca + 2 containing Krebs saline was applied to the nerve for 3 min (Figure 1E). PEG average molecular weight used was 200 Da. Due to the use of PEG with average molecular weight of 200 Da, the method used would be better referred as "PEG-Fusion experience", since the so-called "PEG-Fusion protocol" described by Bittner et al. (2012) used a 2 k Da molecular weight of PEG. There are recently published papers describing that the molecular weight of PEG may interfere with the results. The authors would like to apologize for any inconvenience caused.

Distilled water

Methylene blue

10.1002/hed.25626

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Antipsychotic in the treatment of acute mania: A retrospective study at centro hospitalar psiquiátrico de lisboa

European Neuropsychopharmacology (2019)

Silvia Nascimento J. Aguiar Inês Morais Caldas Carla Pereira Marta Nascimento

Cued speech

10.1016/j.euroneuro.2018.11.562

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New Experimental Model of Facial Nerve Function and Integrity to Evaluate Regeneration

Otolaryngology (2014)

Ricardo Ferreira Bento Heloisa Juliana Zabeu Rossi Costa Silvia Nascimento Ricardo José Rodriguez Ferreira Raquel Salomone

Objectives: The ideal animal model for nerve regeneration studies is an object of controversy because all models described by the literature have advantages and disadvantages. Small animals such as rats have been repeatedly used in experimental procedures because of their easy caretaking and handling, resistance to manipulation and infections, and low cost. In this case, rats have the additional benefit of tolerating bilateral facial paralysis. This study aimed to describe the histologic and functional (electroneuromyographic) patterns of the mandibular branch of the facial nerve of Wistar rats in order to create a new experimental model of facial nerve regeneration. Methods: Forty‐two male Wistar rats were submitted to neurophysiologic evaluation by a nerve conduction test of the mandibular branch of the facial nerve to obtain the compound muscle action potential. Twelve of these rats had the mandibular branch of the facial nerve surgically removed and submitted to histologic analysis (total axon number, partial axonal density, and axonal diameter) of the proximal and distal nerve segments. Results: There was no statistical difference in the functional and histologic variables studied. Conclusion: These new histologic and functional standards of the mandibular branch of the facial nerve of rats establish an objective, easy, and greatly reproducible model for future facial nerve regeneration studies.

Facial muscles

Facial paralysis

10.1177/0194599814541629a16

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CHONDROBLASTOMA OF THE TEMPORAL BONE: CASE REPORT

International Archives of Otorhinolaryngology (2012)

Silvia Nascimento Ana Almeida Euler Silva

Chondroblastoma

10.7162/s1809-977720120s1po-081

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Evaluation of the Frequency and Causes of Newborn Hearing Screening Failure in a Private Hospital in Teresina, Piauí

International Archives of Otorhinolaryngology (2014)

Renê Lima Porto Danusa Oliveira Gisa Carvalho Rayra Barbosa Silvia Nascimento

Introduction: The Brazilian Committee on Hearing Loss in Childhood (CBPAI) and the Multidisciplinary Committee on Hearing Health (COMUSA) recommend the implementation of the Universal Neonatal Hearing Screening (UNHS) in all newborns at birth or until 3 months of age.

Multidisciplinary team

10.1055/s-0034-1388676

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Sutura química por polietilenoglicol na regeneração do nervo facial em ratos após neurotmese

Silvia Nascimento

BACKROUND:The gold standard treatment for traumatic transection of the FN continues to be end-to-end anastomosis using fibrin glue, which often yields unsatisfying results.OBJECTIVE: To test the outcome of a novel method of polyethylene glycol (PEG)-fusion on FN transection using electrophysiological and histophormometric parameters.METHODS: Wistar rats were divided into 4 groups.After FN transection, the control group was submitted to end-to-end anastomosis with microsutures.Group 2 was submitted to microsutures plus the PEG-fusion protocol.This protocol consisted in bathing nerve stumps with a calcium-free Krebs solution containing methylene blue (MB) before suturing.After suturing, the repaired nerve received a PEG solution followed by a calciumcontaining Krebs solution.Group 3 received microsutures plus the MB solution and group 4 received microsutures plus the PEG solution.Compound muscle action potentials (CMAPs) were recorded before the intervention and 3 and 6 weeks afterwards.Histomorphometric analysis was done at 6 weeks time. RESULTS:The PEG-fusion protocol yielded larger CMAP amplitude, smaller CMAP duration at 3 and 6 weeks and a larger axon count and axon diameter.Between the other groups, no significant difference was seen.CONCLUSION: PEG-fusion produces better FN recovery after transection, when considering electrophysiological and histomorphometric analysis and may be of use in clinical scenarios of FN cut-severance followed by immediate repair.

10.11606/t.5.2018.tde-14032018-121636

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