Intracellular deoxyribonucleotide pools were examined before and after thymidine treatment in highly sensitive T-lymphoid cells, relatively resistant B-lymphoid cells and moderately sensitive melanoma cells. Among the 4 cell lines studied, proportions of the 4 deoxyribonucleotide pools varied appreciably while ribonucleotide profiles were similar. The ratio of dGTP to dCTP increased with sensitivity to thymidine. Increase in dTTP levels with increasing thymidine concentration was dependent on sensitivity of cells to thymidine and was accompanied by reduction in the dCTP pool. dGTP levels increased as did dTTP levels in all cells, while dATP pool expansion correlated with thymidine sensitivity. The results indicate an additional aspect of purine deoxyribonucleotide involvement in the growth inhibitory effects of thymidine.
The discovery that angiogenesis is a key condition for the growth of a tumor beyond a millimeter or two, brings about a new approach in the treatment of tumors using drugs able to inhibit the formation of new blood vessels. Also, it has been realized that antiangiogenic drugs can be useful in the treatment of other pathological processes, now classified as angiogenesis-dependent diseases. Initially, cartilage was considered as a possible natural source of antiangiogenic compounds due to its known avascular nature. To date, a number of in vitro and in vivo studies have suggested the existence of antiangiogenic and antitumor compounds in bovine and shark cartilage. However, the potential usefulness of shark cartilage in the treatment of cancer and other angiogenesis-dependent diseases have not been totally accepted due to (i) unsatisfactory patient outcome in clinical trials that have used shark cartilage in cancer patients, (ii) the lack of data that correlates bioavailability with pharmacological effects using oral shark cartilage. Thus, the objective of this review is to describe the main basic and clinical investigations reported in the literature, in which the antiangiogenic and/or antitumor properties of shark cartilage or of its extracts were evaluated. Possible explanations for conflicting results are discussed as well.
Journal Article Analysis of Human Ascites Effect on Clonogenic Growth of Human Tumor Cell Lines and NRK-49F Cells in Soft Agar Get access H. J. Broxterman, H. J. Broxterman Department of Oncology, Free University Hospital, Amsterdam, The Netherlands Correspondence: H. J. Broxterman, Free University Hospital, Department of Oncology, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar C Sprenkels-Schotte, C Sprenkels-Schotte Department of Oncology, Free University Hospital, Amsterdam, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar A. Leyva, A. Leyva Department of Oncology, Free University Hospital, Amsterdam, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar H. M. Pinedo, H. M. Pinedo Department of Oncology, Free University Hospital, Amsterdam, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar P. H. Engelen P. H. Engelen Department of Gynecology, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar The International Journal Of Cell Cloning, Volume 5, Issue 2, 1987, Pages 158–169, https://doi.org/10.1002/stem.5530050208 Published: 01 January 1996 Article history Received: 11 April 1986 Accepted: 05 September 1986 Published: 01 January 1996
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