Background: Invasive prenatal evaluation by chromosomal microarray analysis (CMA) and karyotyping might represent an important option in pregnant women, but limited reports have applied CMA and karyotyping of fetuses conceived by assisted reproductive technology (ART).This study aimed to examine the value of CMA and karyotyping in prenatal diagnosis after ART.Methods: This retrospective study included all singleton fetuses conceived by ART from January 2015 to December 2021.Anomalies prenatally diagnosed based on karyotyping and CMA were analyzed.Prevalence rates for various CMA and karyotyping results were stratified based on specific testing indications including isolated -and non-isolated ART groups.The rates of CMA findings with clinical significance (pathogenic/likely pathogenic) and karyotype anomalies were assessed and compared to those of local control individuals with naturally conceived pregnancies and without medical indications.Results: Totally 224 subjects were assessed by karyotyping and CMA.In the examined patients, chromosomal and karyotype abnormality rates were 3.57% (8/224) and 8.93% (20/224), respectively.This finding indicated a 5.35% (12/224)-incremental rate of abnormal CMA was obtained over karyotype analysis (p=0.019).The risk of CMA with pathogenic findings for all pregnancies conceived by ART (5.80%, 13/224) was markedly elevated in comparison with the background value obtained in control individuals (1.47%, 9/612; p=0.001).In addition, risk of CMA with clinically pathogenic results in isolated ART groups was significant higher compared to the background risk reported in the control cohort (p = 0.037).Conclusions: Prenatal diagnosis including karyotyping and CMA is recommended for fetuses conceived by ART, with or without ultrasound findings.
Nowadays robotic technology is widely used in rehabilitation especially in stroke rehabilitation. Comparing conventional robots, cable-driven parallel robots have many advantages such as low moving inertia, high power efficiency, large workspace, low cost, and so forth. On this account, three-degree-of-freedom (3-DOF) cable-driven parallel for upper limb rehabilitation is currently under development. This paper presents motion analysis of upper limb to design a 3-DOF upper limb rehabilitation robot. Main feature of this rehabilitation robot is to provide the relatively large workspace and to be less dangerous in the situation of robot's malfunction due to reduced moving part. Since our rehabilitation is being designed to have a planar workspace, the motion of upper limb is also analyzed in a plane.
In a simulation study evaluating the residual effects of taking a single night-time dose of hypnotics among health individuals on collision anticipation capacities in the next morning, investigator ...
In the pathogenesis of age-related macular degeneration, long non-coding RNAs have become important regulators. This study aimed to investigate the role of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in the progression of choroidal neovascularization (CNV) and the underlying mechanisms. The in vivo and in vitro model of CNV was established using laser-induced mouse CNV model and human choroidal vascular endothelial cells (HCVECs) exposed to hypoxia respectively. We explore the role of MALAT1 in the pathogenesis of CNV by using the small interference RNA both in vivo and in vitro. MALAT1 expression was found to be upregulated in the retinal pigment epithelial-choroidal complexes. MALAT1 knockdown inhibited CNV development and leakage in vivo and decreased HCVECs proliferation, migration, and tube formation in vitro. MALAT1 performed the task as a miR-17-5p sponge to regulate the expression of vascular endothelial growth factor A (VEGFA) and E26 transformation specific-1 (ETS1). This study provides a new perspective on the pathogenesis of CNV and suggests that the axis MALAT/miR-17-5p/VEGFA or ETS1 may be an effective therapeutic target for CNV.
Deyeuxia angustifolia acts as an indicator of the changes in wetland ecosystems and plays an important ecological role in their functions. Previous studies have shown that the Tumen River Basin is of wide global interest as a transboundary basin area with abundant wetland ecological resources. Despite the implementation of wetland conservation measures in recent years, the distribution area of D. angustifolia in this basin has been reduced due to climate change and anthropogenic activities. Therefore, we used MaxEnt and geographic information system to model a suitable habitat for this species, simulated changes in the habitat, and applied Jackknife to assess the influencing environmental variables. The Jackknife tests showed that slope orientation, solar radiation in September, and total nitrogen were the dominant environmental factors affecting the potential distribution of D. angustifolia . Compared to the current distribution, the total area of land highly and moderately suitable for this species will decrease in the 2050’s and 2070’s, respectively, under two representative concentration paths (RCPs), shifting the centroid of its suitable area and direction of wetland degradation to the northeast. Our study of the projected potential distribution of D. angustifolia in the basin under future climate change could provide important information for its conservation, management, sustainable use, and early warning to prevent its extinction.
Objective
To explore the value of comprehensive management and rehabilitation therapy in the treatment of patients with stable COPD.
Methods
Selected March 2014-August 2015 up to the discharge standard hospital respiratory medicine 70 patients with stable COPD patients, by using the method of random number table, 35 cases were divided into study group and control group.Two groups were given routine medical advice, the study group also under the guidance of the researchers to carry out the comprehensive management of pulmonary rehabilitation based on rehabilitation treatment, the intervention time of 6 months.
Results
Before intervention, the study group and control group FEV1, FEV1%pred, 6 min walking distance, CAT score, mmMRC score, BODE index were not statistically significant (P>0.05). At 12 weeks and the end of the intervention, the FEV1, FEV1%pred and 6 min walking distance of the study group were significantly higher than those of the control group (P<0.05), the CAT score, mMRC score and BODE index in the study group were significantly higher than those in the control group (P<0.05). During the intervention period, the average AECOPD times of the study group was [(0.59±0.22) times] significantly lower than that of the control group [(0.92±0.30) times](t=5.928, P<0.05).
Conclusions
Pulmonary rehabilitation combined with rehabilitation treatment can improve the lung function and quality of life of COPD patients, and reduce the number of AECOPD.
Key words:
Pulmonary rehabilitation; Integrated management; Rehabilitation; Stable; Chronic obstructive pulmonary disease
In wheat, a series of dwarf and semi-dwarf plant varieties have been developed and utilized worldwide since the 1960s and caused the ‘Green Revolution’. To date, 25 reduced-height (Rht) genes have been identified, but only several genes for plant height (PH) have been isolated previously. In this study, we identified a candidate gene, ATP-dependent DNA helicase (TaDHL-7B), for PH via QTL mapping and genome-wide association study (GWAS) methods. We knocked out this gene using the CRISPR/Cas9 system in variety ‘Fielder’. Two homozygous mutant genotypes, AAbbDD (−5 bp) and AAbbDD (−1 bp), were obtained in the T2 generation. The PH values of AAbbDD (−5 bp) and AAbbDD (−1 bp) were significantly reduced compared with the wild-type (WT, ‘Fielder’), indicating that TaDHL-7B is a novel Rht gene that controls the PH. This is the first time that a PH gene of wheat has been isolated with a non-hormone pathway, providing a new insight into the genetic control of PH. The TaDHL gene reduced the PH without a yield penalty. It could be used to improve the lodging resistance and yield in wheat breeding programs.
In order to detect the sleep spindles simply and efficiently, a novel time-domain approach to detect sleep spindles based on the principles of visual organization is proposed. The code idea of the visual organization is to organize the primary visual elements according to some rules of organization, and to form a more meaningful object of visual processing, as the input of next process. After the collected EEG is processed with the merging algorithm based on the principle of visual organization, it can extract the time-domain feature frequency and duration time better. Use these features with a simple algorithm to detect spindles achieving sensitivity of 92.5% and specificity of 98.1%, which verifies the validity of this method to detect the sleep spindles.
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