Objective To study the effect of aerobic and anaerobic training on the serum levels of free radicals. Methods 26 middle and long distance athletes were selected from the track and field team of Shanghai City and Shanghai Institute of Physical Education and divided into two groups (12 for aerobic training and 14 for anaerobic training). Every athlete was trained using a specific training program designed according to the athlete's ability. Blood was drawn from the vein before and soon after the training. Biochemistry tests which were associated with the production and cleaning of free radicals including the level of serum ROS, SOD, CAT and lactic acid were detected. Results ①The serum level of LD and Na~+ were significantly increased and the serum level of Cu was significantly decreased in both groups after training(p0.01).② The serum level of SOD was significantly decreased(p0.01)and the serum level of Mg~ ++ was decreased (p0.05)in aerobic training group. ③ In anaerobic training group, the serum level of CAT was decreased(p0.05), and the serum level of Ca and Fe were significantly increased (p0.01). ④ Compared with aerobic training group, the serum level of LD, SOD,Na~+ and Fe in anaerobic training group were increased, and the serum level of potassium was decreased. Conclusions ① The effects of aerobic and anaerobic training on the ability of cleaning plasma free radical are different. ② The decreased level of copper after training affects the activity of superoxide dismutase and the cleaning of free radical in the plasma.
CD300A is a type I transmembrane receptor protein which has shown inhibitory effects on B-cell receptor-mediated signals. In an analysis of public dataset, we found that CD300A mRNA levels were inversely correlated with the overall survival time of patients with diffuse large B-cell lymphoma (DLBCL). To decipher the role of CD300A in DLBCL, we knocked down the expression levels of CD300A in DLBCL cells and found that decreasing levels of CD300A significantly inhibited cell proliferation of OCI-Ly01, Farage, and SUDHL-4 cells, but not of VAL, OCI-Ly10, or SUDHL-8 cells. Mechanistically, reduced expression of CD300A resulted in a marked attenuation of AKT phosphorylation, a key molecular event in tumorigenesis, in OCI-Ly01, Farage, and SUDHL-4 cells. Pharmacologic inhibition of PI3K displayed a similar inhibitory effect on cell proliferation. Furthermore, using a xenograft animal model, we found that decreasing levels of CD300A in OCI-Ly01 and Farage cells significantly inhibited tumor formation in vivo. Collectively, our results suggested an oncogenic role of CD300A in DLBCL which could serve as a potential biomarker and therapeutic target for this malignant disease.
Abstract Motivation Circular RNAs (circRNAs) have been found to have the potential to code proteins. Internal ribosome entry sites (IRESs) are key RNA regulatory elements for the translation of proteins by circRNAs through a cap-independent mechanism. IRES can be identified by bicistronic assay, but the method is time-consuming and laborious. Therefore, it is important to develop computational methods for facilitating IRES identification, evaluation, and design in circRNAs. Results In this study, we proposed DeepCIP, a multimodal deep learning approach for circRNA IRES prediction, by exploiting both sequence and structure information. As far as we know, DeepCIP is the first predictor for circRNA IRESs, which consists of an RNA processing module, an S-LSTM module, a GCN module, a feature fusion module, and an ensemble module. The comparative studies show that DeepCIP outperforms other comparative methods and justify the effectiveness of the sequence model and structure model of DeepCIP for extracting features. We found that the integration of structural information on the basis of sequence information effectively improves predictive performance. For the real circRNA IRES prediction, DeepCIP also outperforms other methods. DeepCIP may facilitate the study of the coding potential of circRNAs as well as the design of circRNA drugs. DeepCIP as a standalone program is freely available at https://github.org/zjupgx/DeepCIP .
Microbial dysbiosis has an increasingly appreciated impact on carcinogenesis and cervicovaginal microbiome plays a critical role in microenvironmental inflammation. Here we investigated the involvement of the female genital tract microbiome in a gynaecological cancer. We transferred Peptostreptococcus species into the uteri of BALB/c nude mice and observed indoleacrylic acid (IAA) production. Intratumoral injection of conditioned medium from bacterial cultures into tumour-xenografted mice for 2 months promoted tumour growth. IAA upregulated IL-10 expression by M2 macrophages, which increased IFN-γ expression by CD8+ T cells. IFN-γ induced indoleamine-2,3-dioxygenase 1 (IDO1) expression in endometrial cancer (EC) cell line. Co-culture of IDO1-expressing EC cells with peripheral blood mononuclear cells upregulated the proportion of regulatory T cells and decreased the M1/M2 ratio. Importantly, P. anaerobius was more abundant amongst the uterine microbiota of clinical EC patient samples than control samples. IAA, IDO1, and the kynurenine/tryptophan ratio were all higher in EC tissue than in healthy tissue, and the M1/M2 ratio was lower. Thus, we conclude that the overabundance of uterine commensal Peptostreptococcus species in EC induces IDO1 expression by producing IAA. Our study sheds light on the link between IDO1 induction and uterine Peptostreptococcus dysbiosis.
Fruit ripening is usually accompanied by anthocyanin accumulation. Ethylene is key in ripening-induced anthocyanin production in many fruits. However, the effects of fruit ripening and ethylene on anthocyanin biosynthesis in purple tomato fruits are unclear. This study shows that bagged fruits of the purple tomato cultivar 'Indigo Rose' failed to produce anthocyanins at the red ripening stage after bag removal. In contrast, the bagged immature fruits accumulated a significant amount of anthocyanins after removing the bags. The transcriptomic analyses between immature and red ripening fruit before and after bag removal revealed that anthocyanin-related genes, including the key positive R2R3-MYB regulator SlAN2-like, were repressed in the red ripening fruit. The 86 identified transcription factors, including 13 AP2/ERF, 7 bZIP, 8 bHLH and 6 MYB, showed significantly different expressions between immature and red ripening fruits. Moreover, subjecting bagged immature fruits to exogenous ethylene treatment significantly inhibited anthocyanin accumulation and the expression of anthocyanin-related genes, including the anthocyanin structure genes and SlAN2-like. Thus, ethylene inhibits anthocyanin biosynthesis by repressing the transcription of SlAN2-like and other anthocyanin-related genes. These findings provide new insights into anthocyanin regulation in purple tomato fruit.
To investigate the abnormalities of the relative factors of pRb-p16 cell regulatory pathway in pancreatic adenocarcinoma.Five strains of human pancreatic adenocarcinoma cell lines were analysed by PCR and single-strand conformation polymorphism (SSCP) for homozygous deletion and mutation of the p16 gene, respectively. The gene amplification of cyclin D1 was detected by Southern blot analysis and the protein expression of pRb gene was examined by Western blot analysis.Homozygous deletion of exon 1 were found in two cell lines and no mutation was found within exon 1 and exon 2 in five cell lines. Cyclin D1 gene amplification was observed in one cell line and all of the five cell lines showed hyper-phosphorylated pRb protein.These findings have demonstrated that there are abnormalities of pRb-p16 regulatory pathway in pancreatic adenocarcinoma cell lines, revealing the alteration of the p16 and cyclin D1 genes.
Background: During the crisis of communicable diseases, nurses play an important role in controlling the disease and treating patients; therefore, the obstacles and challenges facing these medical personnel should be reduced. Objectives: The main goal of the present study was to evaluate the challenges faced by nurses during the Coronavirus Disease 2019 (COVID-19) pandemic. Methods: This qualitative study was conducted using the content analysis approach in 2021-22 in the city ABC. The participants in this study were 30 people who were selected from 10 different hospitals and included nursing managers (n=10), supervisors (n=10), and nurses (n=10). Sampling was done purposefully and continued until the information saturation point. Semi-structured in-depth interview was used individually based on the interview guideline to collect data. Results: The results showed that the challenges faced by nurses during the COVID-19 pandemic included 452 codes and 12 subcategories, which after the final classification of the data, 4 main challenge categories were obtained, which included human challenges (33 subcategories), financial challenges (15 subcategories), communication challenges (8 subcategories), and organizational challenges (27 subcategories). Conclusion: Based on the results of the present study, it can be concluded that the challenges faced by nurses during the COVID-19 pandemic included human, financial, communication, and organizational obstacles. Recognizing the challenges can help formulate road maps and strategies for improving disaster preparedness and management in hospitals.
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