Eupatorium adenophorum Spreng can potentially be utilized in several bioenergy pathways. When pretreated with microbial inoculum, it can be used as biogas fermentation material, which could increase the biogas production velocity. Eupatorium adenophorum Spreng is a promising potential biogas feedstock, whose rate of gas generation is 180 (m3-t_1TS) and fuel conversion efficiency is 12.37% at 30°C. When co-fermentated for hydrogen and methane production, it can increase the material degradation velocity and energy utilization efficiency, and decrease the fermentation reaction period.
Retinoblastoma (RB) is a malignant tumor that is derived from photoreceptors. It is common in children under 3 years old with a family genetic predisposition. MicroRNA-133a-3p (miR-133a-3p) is one of the tumor-related miRNAs that interprets a critical function in the genesis and development of various tumors. This study investigated the effects and underlying mechanisms of miR-133a-3p in RB.Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was used to assess the miR-133a-3p expression in RB tissues and a cell model. MTT assay, western blot, flow cytometry and luciferase reporter assay were performed to evaluate the effect of miR-133a-3p on cell viability, apoptosis and the cell cycle. An RB xenograft model was established to assess the in vivo influence of miR-133a-3p on RB growth.MiR-133a-3p level was reduced in RB tissues and the cell model (p < 0.01 or p < 0.001). Addition of miR-133a-3p reduced cell viability, and increased apoptosis and cell cycle arrest (p < 0.001). Additionally, CREB1 was identified to be the target of miR-133a-3p in RB cell lines (p < 0.001). Cell viability reduction, apoptosis and cell cycle arrest increases mediated by miR-133a-3p were attenuated by CREB1 overexpression (p < 0.001). MiR-133a-3p inhibited tumor growth of RB in vivo (p < 0.001).Our results reveal that miR-133a-3p exhibits anti-cancer effects by targeting CREB1 in RB. This study provides a new direction for effective targeted treatment of this disease.
Objective To screen siRNAs that can effectively inhibit Apollon gene expression and determine the cellular functions of those siRNAs.Methods A chemical synthesis method was used to synthesize 3 siRNA sequences against different sites of Apollon.They were transfected into the human breast cancer MCF-7 cells by using Lipofectamine 2000.mRNA level of Apollon was determined by reverse transcription-polymerase chain reaction (RT-PCR).Cellular immunity fluorescence quantitative analysis combined with confocal laser technology was used to determine the protein level of Apollon.Methyl thiazolyl tetrazolium bromide (MTT) assay and flow cytometry were used to determine the effects of siRNA targeting Apollon on proliferation and apoptosis of MCF-7 cells,respectively.Results Three pairs of siRNA could significantly inhibit Apollon mRNA expression,at the inhibition rates of (36.201±11.629) ％,(67.308±7.686) ％and (47.123±12.000) ％,respectively (P ＜ 0.05).After tranfection by siRNA2,Apollon protein fluorescence intensity was (14.97±2.08) ％ compared with control cells.The cell proliferation MCF-7 was inhibited by (73.361±2.118) ％and apoptosis was increased by (28.793±0.743) ％.Conclusions Screened siRNA2 effectively silences Apollon gene expression,effectively inhibits the proliferation and increases the apoptosis of MCF-7 cells.This provids the foundation for its clinical application in cancer therapy.
Key words:
Neoplasms; Apollon gene; RNA, small interfering; MCF-7 cell
Objective
To explore the prognostic value of N-terminal pro-brain natriuretic peptide(NT-pro-BNP) levels in critically ill infants.
Methods
Eighty-one critically ill infants were enrolled from January 2013 to January 2014 in pediatric intensive care unit.The minimum of pediatric critical illness score(PCIS) and the number of dysfunction organs were calculated within 24 hour after admission.According to PCIS, the critically ill infants were divided into extremely critical group(PCIS≤70, n=25), critical group(PCIS 71-80, n=30)and non-critical group(PCIS>80, n=26). According to the prognosis, the critically ill infants were divided into survival group(n=68)and death group(n=13). The serum NT-pro-BNP levels were determined on the first day, third day and convalescent phase.The relationships of serum NT-pro-BNP levels with PCIS and the number of dysfunction organs and prognosis were observed.
Results
The study showed statistical significances of serum NT-pro-BNP levels among the extremely critical group, critical group and non-critical group, whether on the first day, or on the third day and convalescent phase(P<0.01). There were statistical significances of serum NT-pro-BNP levels among different stages of the disease in each group(P<0.01). Compared with survival group, PCIS was significantly lower and the serum NT-pro-BNP levels and the number of dysfunction organs were significantly higher in death group.The serum NT-pro-BNP level on the third day was higher than that on the first day in death group(P<0.01), while no significant difference was found in survival group.The serum NT-pro-BNP levels on the first day and the third day and PCIS were negatively correlated(r=-0.59, P<0.01; r=-0.66, P<0.01). The serum NT-pro-BNP levels on the first day and the third day and the number of dysfunction organs were positively correlated(r=0.40, P<0.05; r=0.57, P<0.01).
Conclusion
The serum NT-pro-BNP levels of the critically ill infants are correlated with disease severity, and can be useful for assessing the severity of critical illness.
Key words:
Critical illness; Infants; N-terminal pro-brain natriuretic peptide; Prognosis
Matrix metalloprotease 12 plays a significant role in airway inflammation and remodeling. Increased expression and production of MMP-12 have been found in the lung of human COPD patients. MMP408 (14), a potent and selective MMP-12 inhibitor, was derived from a potent matrix metalloprotease 2 and 13 inhibitor via lead optimization and has good physical properties and bioavailability. The compound blocks rhMMP-12-induced lung inflammation in a mouse model and was advanced for further development for the treatment of COPD.
Objective To investigate the effects of small interference RNA (siRNA) targeting HOXA9 on the proliferation and apoptosis of human acute monocytic leukemia U937 cell line.Methods Effective and specific siRNA oligo targeting HOXA9 was designed and compounded.It was transfected transiently into U937 cells by cationic liposome.The cells was divided into three groups:experimental group(siRNA targeting HOXA9 was transfected by liposome),negative control group (negative siRNA was transfected by liposome) and cell control group (add equal cells and medium).The expression of HOXA9 mRNA and protein were detected by reverse transcription PCR and Western blot.The cell proliferation was assessed by MTT.The apoptosis of each group were measured by Annexin V-FITC.Results Aftcr transfected by siRNA targeting HOXA9,the relative mRNA expression levels of HOXA9 in the experimental group,negative control group and cell control group were (22.980±0.548) ％,(82.371±1.517) ％ and (84.637±2.252) ％,respectively (P ＜ 0.05),and the relative protein expression levels were (50.377±2.773).％,(105.500±3.900) ％ and (111.392±3.905) ％,respectively (P ＜ 0.05).The inhibitory rates of cell proliferation and the apoptosis rates of the experimental group were significantly increased.The inhibitory rates of cell proliferation of 24 h,48 h and 72 h were (41.909±4.333) ％,(54.470±3.756) ％ and (65.835±1.024) ％,respectively,and the apoatosis rate was (26.800±2.081) ％.Compared with 2 controls,the experimental group differences had statistically significance (P ＜ 0.05).Conclusion siRNA targeting HOXA9 can effectively silence HOXA9 gene expression in U937 cell,suppress cell proliferation and induce cell apoptosis obviously,which providing experimental basis for clinical lenkemia therapy by targeting HOXA9 gene.
Key words:
Genes, Homeobox A9; Leukemia; RNA interference; U937 cells
Objective
To investigate the possible mechanism of Th9 cells and their cytokine interleukin-9(IL-9) in children with immune thrombocytopenia (ITP).
Method
42 Children with ITP in the department of heamatology and Neurology of children in the affiliated hospital of Binzhou medical college from August 2014 to January 2015 were selected as experimental subjects, In the same period, 30 healthy children were selected as control. There were no significant differences in gender and age between the two groups of children. Th9 cells in peripheral blood were measured and IL-9 cytokine detection was done.
Result
The difference of the proportion of Th9 cells in the peripheral blood of the two groups was significant different. The levels of IL-9 in peripheral blood of the experimental group were higher than those in the control group.
Conclusion
Th9 cells and IL-9 may be involved in promoting the proliferation and activation of effector cells, such as T, B lymphocytes and mast cells, to participate in immune regulation.
Key words:
Th9 cell; Interleukin-9; Immune thrombocytopenia
Objective To explore the anti-tumor effects of Herceptin and mitomycin C (MMC) on human bladder urothelial carcinoma T24 lines. Methods The SP immunohistochemical method and RT-PCR method were used to detect the expression of HER-2/neu in human bladder urothelial carcinoma T24 lines. MTT method was applied to assay the growth inhibition rate of various concentrations of Hercep-tin (10,20,40,80,and 160 μg/L),MMC (4,8,16,32,and 64 μg/L),Herceptin combined with MMC on human bladder urothelial carcinoma T24 lines. Results There was high expression of HER-2/neu in hu-man bladder urothelial carcinoma T24 lines by immunocytochemistry and RT-PCR. Herceptin slightly in-hibited proliferation of human bladder urothelial carcinoma T24 lines at the 72nd h as compared with that at the 48th h, all P<0.05). The combined use of herceptine and MCC exerted the synergistic effects at the 24th,48th,and 72nd h (q value: 1. 264 to 3. 473),and an additive effect was obtained at the 96th h (q value:0.913 to 1. 138). Conclusion Her-2/neu was overexpressed in human bladder urothelial carcino-ma T24 lines. Herceptin slightly inhibited proliferation of T24 lines and exerted the effects slowly. There was a synergistic effect to inhibit proliferation of T24 lines by combined use of herceptine and MMC.
Key words:
Bladder carcinoma; Herceptin; Gene expression; Mitomycin
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