The study was conducted at Fatki River under Magura district to learn the aquatic weeds diversity and abundance. This paper focused on species variation, number of species, water quality parameter and identification of aquatic weeds. It was assessed by collecting samples from Chukinogor Ghat, Kechuadubi and Arpara bazar during May to August, 2016. Aquatic weeds were abundant in rainy season and the semi-aquatic weeds were available almost all the year round. According to this study, total 22 species belonging to 12 orders, 16 families and 21 genuses were found. Again, 46%, 28%, 26% of total aquatic weeds percentages identified from the Chukinogor Ghat, Kechuadubi and Arpara bazar respectively. During this study period, nine species of family Pontederiaceae, Convolvulaceae, Menyanthaceae, Asteraceae, Poaceae, Araceae, Amaranthaceae, Polygonaceae were common in Chukinogor Ghat, Kechuadubi and Arpara bazar. Noticeable species– Lemna minor, Marsilea quadrifolia, Ludwigia palustris, Aeschynomene aspera, Najas graminea, Hydrilla verticillata, Najas guadalupensis, Utricularia inflate were only found in Chukinogor Ghat. This species are easily adapted in large areas. On the other hands, two species were common in Chukinogor Ghat, Kechuadubi – Pistia stratiotes and Equisetum hyemale. Prominent species of Arpara bazar were Cyperus rotundus and Xanthium indicum. Total 11 species of aquatic weeds were mainly recognized from these Kechuadubi, while greatest number was recorded of 20 species from Chukinogor Ghat and 12 species were have its place to Arpara bazar. Management technique and water quality parameters were also studied during study period and the temperature was recorded 29.0-30.00 oC in Chukinogor Ghat, same as 28.0-320 oC in Kechuadubi. Furthermore, 29.0-31.00 oC temperature was found in Arpara bazar. DO levels were stable at ranged from7.0-7.5 ppm in Chukinogor Ghat and 4.7-5.3 ppm was recorded in two experimental Kechuadubi 4.5-5.0 in Arpara bazar respectively. This difference may arise due to the using of aerator in the Chukinogor Ghat same as decomposition, intensity of light in Kechuadubi and Arpara bazar. Transparency was 28-32cm in Chukinogor Ghat, 36-44cm in Kechuadubi, and 38-42 cm in Arpara bazar.
A cross sectional study was carried out from June, 2008 to December, 2010 to estimate the prevalence of subclinical mastitis and to determine the responsible bacterial pathogens in lactating dairy goats in northern districts of Bangladesh. A total of 292 goats and 584 udder halves milk samples were screened using California Mastitis Test (CMT). Prevalence of subclinical mastitis at goat level was 56.2% (164/292), and in udder half level it was 33.9% (198/584). The subclinical mastitis prevalence at goat level was high (71.6%) in Jamnapari goats as compared to Black Bengal goats (50.2%), whereas at the udder half level, subclinical mastitis prevalence was 45.1% and 29.6% in Jamnapari and Black Bengal goats, respectively. The pathogens isolated from subclinical mastitic milk samples were coagulase negative Staphylococci, Coliforms, Streptococcus spp., Staphylococcus aureus, Micrococcus spp., Pseudomonas aeruginosa and Bacillus cereus. Among these, the most frequent isolates were coagulase negative Staphylococci (28.8%), Coliforms (22.7%) and Streptococcus spp (15.2%). Out of four potential host related risk factors considered, litter size and body condition of goats were found to influence the prevalence rate of subclinical mastitis in goat significantly (p< 0.05). On the other hand, the subclinical mastitis was very significantly (p=0.0001) associated with the housing system of goats, i.e., goats reared in raised floor had a low subclinical mastitis infection rate (35.8%) as compared to reared in earth floor (62.2%). Antibiogram studies were also performed for the bacterial isolates and Gentamicin was found to be the most effective drug.Bangladesh J. of Livestock Res. 19(1-2): 112-122, Jan-Dec 2012
A genomic study was conducted to identify the effects of urbanization and environmental contaminants with heavy metals on selection footprints in dairy cattle populations reared in the megacity of Bengaluru, South India. Dairy cattle reared along the rural–urban interface of Bengaluru with/without access to roughage from public lakeshores were selected. The genotyped animals were subjected to the cross-population–extended haplotype homozygosity (XP-EHH) methodology to infer selection sweeps caused by urbanization (rural, mixed, and urban) and environmental contamination with cadmium and lead. We postulated that social-ecological challenges contribute to mechanisms of natural selection. A number of selection sweeps were identified when comparing the genomes of cattle located in rural, mixed, or urban regions. The largest effects were identified on BTA21, displaying pronounced peaks for selection sweeps for all three urbanization levels (urban_vs_rural, urban_vs_mixed and rural_vs_mixed). Selection sweeps are located in chromosomal segments in close proximity to the genes lrand rab interactor 3 (RIN3), solute carrier family 24 member 4 (SLC24A4), tetraspanin 3 (TSPAN3), and proline-serine-threonine phosphatase interacting protein 1 (PSTPIP1). Functional enrichment analyses of the selection sweeps for all three comparisons revealed a number of gene ontology (GO) and KEGG terms, which were associated with reproduction, metabolism, and cell signaling-related functional mechanisms. Likewise, a number of the chromosomal segments under selection were observed when creating cattle groups according to cadmium and lead contaminations. Stronger and more intense positive selection sweeps were observed for the cadmium contaminated group, i.e., signals of selection on BTA 16 and BTA19 in close proximity to genes regulating the somatotropic axis (growth factor receptor bound protein 2 (GRB2) and cell ion exchange (chloride voltage-gated channel 6 (CLCN6)). A few novel, so far uncharacterized genes, mostly with effects on immune physiology, were identified. The lead contaminated group revealed sweeps which were annotated with genes involved in carcass traits (TNNC2, SLC12A5, and GABRA4), milk yield (HTR1D, SLCO3A1, TEK, and OPCML), reproduction (GABRA4), hypoxia/stress response (OPRD1 and KDR), cell adhesion (PCDHGC3), inflammatory response (ADORA2A), and immune defense mechanism (ALCAM). Thus, the findings from this study provide a deeper insight into the genomic regions under selection under the effects of urbanization and environmental contamination.
The current study was carried out to identify the bacterial species in the faecal samples of 20 diarrhoeic sheep and to observe their sensitivity to different antibiotics. This investigation was performed by collecting diarrhoeal samples from the sheep (n=20) under goat and sheep research farm of Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, Bangladesh. The average age and body weight of the animals were 25 days and 2.5 kg respectively. Of the bacteria responsible for diarrhoea in case of sheep, Escherichia coli alone was found in 6 samples (30%), Escherichia coli combined with Proteus mirabilis was found in 12 samples (60%), and no bacteria could be detected in 2 of the 20 samples tested. Both the bacteria were highly sensitive to ceftriaxone, and moderately sensitive to ciprofloxacin and gentamicin. It could be stated that ceftriaxone is the antibiotic of first choice for the treatment of diarrhoea in sheep, where Escherichia coli is suspected to be the principal causal agent of diarrhoea.Res. Agric., Livest. Fish.2(3): 453-457, December 2015
The study was undertaken during the period from July to November 2005, for the first time, to determine the efficacy of thermostable PPR vaccine incubated at -20°C and room temperature (RT) for 14 days which was developed by Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, in collaboration with and Livestock Research Institute (LRI), Mohakhali, Dhaka. A total of 80 healthy goats of both sexes, aged between 8 to 36 months were divided into three groups as A, B and C. Group A and B comprised of 30 goats each and received thermostable PPR vaccine kept at -20°C and at room temperature (RT) respectively for 14 days, at a dose rate of 1 ml (4 Log10 TCID50 / ml) per goat subcutaneously while group C comprising 20 goats served as non-vaccinated control. At 30 days postvaccination, 3 goats from each vaccinated and non-vaccinated group were challenged with 1 ml (5 GID50) of virulent PPR virus per goat subcutaneously in the mid cervical region. Sera samples were collected from all the groups at pre- and post-vaccination (15 and 30 days postvaccination) and were tested for anti-PPR antibody by competitive enzyme linked immunosorbent assay (C-ELISA). In case of group A, average percent inhibition (PI) values of the sera samples at prevaccination and at 15- and 30 days post vaccination were 11.0 ± 5.7, 62.8 ± 9.9 and 81.5 ±5.9 respectively and in case of group B, were 10.0 ± 4.1, 60.7 ± 9.4 and 79.3 ± 7.2 respectively, which were significantly (p < 0.01) higher than those of non-vaccinated control goats (6.3 ± 3.4, 10.2 ± 3.3 and 13.6 ± 3.7 respectively). The titres of vaccinated goats gradually increased until 30 days postvaccination. The challenge test with virulent PPR virus at 30 days post vaccination showed that the thermostable PPR vaccine with both incubation temperatures at -20°C and RT for 14 days protected all the goats (100%) of groups A and B respectively while all the non-vaccinated control goats were not protected after challenge showing clinical signs of PPR and / or mortality. From the above findings it may be concluded that the thermostable PPR vaccine can be kept at normal environmental temperature (25°-30°C) as long as 14 days without loss of its potency and may be used to prevent PPR in goats in field condition.Key Words: Thermostable PPR vaccine, efficacy, C-ELISAdoi:10.3329/bjvm.v4i1.1524 Bangl. J. Vet. Med. (2006). 4 (1): 43-46
A study was designed to identify the genomic regions associated with milk production traits in a dairy cattle population reared by smallholder farmers in the harsh and challenging tropical savanna climate of Bengaluru, India. This study is a first-of-its-kind attempt to identify the selection sweeps for the dairy cattle breeds reared in such an environment. Two hundred forty lactating dairy cows reared by 68 farmers across the rural–urban transiting regions of Bengaluru were selected for this study. A genome-wide association study (GWAS) was performed to identify candidate genes for test-day milk yield, solids-not-fat (SNF), milk lactose, milk density and clinical mastitis. Furthermore, the cross-population extended haplotype homozygosity (XP-EHH) methodology was adopted to scan the dairy cattle breeds (Holstein Friesian, Jersey and Crossbred) in Bengaluru. Two SNPs, rs109340659 and rs41571523, were observed to be significantly associated with test-day milk yield. No significant SNPs were observed for the remaining production traits. The GWAS for milk lactose revealed one SNP (rs41634101) that was very close to the threshold limit, though not significant. The potential candidate genes fibrosin-like 1 (FBRSL) and calcium voltage-gated channel auxiliary subunit gamma 3 (CACN) were identified to be in close proximity to the SNP identified for test-day milk yield. These genes were observed to be associated with milk production traits based on previous reports. Furthermore, the selection signature analysis revealed a number of regions under selection for the breed-group comparisons (Crossbred-HF, Crossbred-J and HF-J). Functional analysis of these annotated genes under selection indicated pathways and mechanisms involving ubiquitination, cell signaling and immune response. These findings point towards the probable selection of dairy cows in Bengaluru for thermotolerance.
A comprehensive study was conducted to assess the effects of seasonal transition and temperature humidity index (THI) on the adaptive responses in crossbred dairy cows reared in a tropical savanna region. A total of 40 lactating dairy cattle reared by small-scale dairy farmers in Bengaluru, India, were selected for this study. The research period comprised the transitioning season of summer to monsoon, wherein all traits were recorded at two points, one representing late summer (June) and the other early monsoon (July). A set of extensive variables representing physiological responses (pulse rate, respiration rate, rectal temperature, skin surface temperature), hematological responses (hematological profile), production (test day milk yield, milk composition) and molecular patterns (PBMC mRNA relative expression of selective stress response genes) were assessed. A significant effect of seasonal transition was identified on respiration rate (RR), skin surface temperature, mean platelet volume (MPV), platelet distribution width (PDWc), test day milk yield and on milk composition variables (milk density, lactose, solids-not-fat (SNF) and salts). The THI had a significant effect on RR, skin surface temperature, platelet count (PLT), plateletcrit (PCT) and PDWc. Lastly, THI and/or seasonal transition significantly affected the relative PBMC mRNA expression of heat shock protein 70 (HSP70), interferon beta (IFNβ), IFNγ, tumor necrosis factor alpha (TNFα), growth hormone (GH) and insulin-like growth factor-1 (IGF-1) genes. The results from this study reveal environmental sensitivity of novel physiological traits and gene expressions to climatic stressors, highlighting their potential as THI-independent heat stress biomarkers.
