Orhan Demir

Harran University

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Gürkan Diken

Isparta University of Applied Sciences

Mehmet Naz

İskenderun Technical University

Erkan Gümüş

Akdeniz University

Süleyman SARIGÖZ

Antalya Eğitim ve Araştırma Hastanesi

Fahretti̇n Küçük

Isparta University of Applied Sciences

Adil Yılayaz

Antalya Eğitim ve Araştırma Hastanesi

Okay Saydam

University of Minnesota Medical Center

Fatih Bulucu

Hitit Üniversitesi Çorum Eğitim ve Araştırma Hastanesi

ÖO

Öxtekin Oto

Dokuz Eylül University

MÖ

Murtaza Ölmez

Ondokuz Mayıs University

Cooperative Institutions

Süleyman Demirel University

Suleyman Demirel University

Isparta University of Applied Sciences

Sağlık Bilimleri Üniversitesi

Karadeniz Technical University

Dokuz Eylül University

University of Minnesota Medical Center

University of Minnesota

Massachusetts General Hospital

Harvard University

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The inhibitory effects of different diets on the protease activities of Argyrosomus regius (Pisces, Scianidae) larvae as a potential candidate species

Journal of Applied Animal Research (2016)

Gürkan Diken Orhan Demir Mehmet Naz

The aim of this research was to gather preliminary data about the potential inhibitory effects of commercial diets on the protease activities of meagre larvae using in vitro techniques. Four commercial diets (Gemma Micro 150 (100–200 µm), Caviar (200–300 µm), Caviar (300–500 µm) and Perla Larva Proactive 4.0 (300–500 µm)) were tested in this study. The differences in the protease activities of meagre larvae during the sampling period were statistically significant (p < .05). The highest and lowest protease activities of meagre larvae were 393.97 ± 7.9 U/mg protein (7 DAH) and 9.64 ± 1.25 U/mg protein (20 DAH), respectively. The digestive proteases of meagre larvae showed the greatest sensitivity to protease inhibitors present in Gemma Micro 150 (100–200 µm). In conclusion, Caviar (200–300 µm), Caviar (300–500 µm) and Perla Larva Proactive 4.0 (300–500 µm) are moderately advisable as the commercial diets in the feeding protocol of meagre larvae from 15 to 32 DAH except for more than 50% inhibitions, but not Gemma Micro 150 (100–200 µm).

10.1080/09712119.2016.1263200

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Citations (5)

Türkiye Su Ürünleri Yetiştiriciliği ve Yem Sektörüne Genel Bakış - II

Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi (2011)

Orhan Demir

Turkiye’de su urunleri yetistiriciligi ile balik yemi uretimine iliskin veriler resmi istatistiklere ilk kayit olarak yakin bir zamanda gecmistir. Ulkemiz yem sanayinde, “balik yemi” adi altinda ilk olarak 1999 yilinda 38 415 tonluk uretimle istatistiklere girmis ve 2010 yilinda 184 810 tona ulasmistir. Su urunleri yetistiriciligine iliskin verilerde ilk olarak 1986 yilinda 3075 tonluk uretim miktariyla kayitlara girmistir. 2010 yilin da 167 141 tona bir baska ifadeyle toplam su urunleri uretiminde % 25,59’lik uretim payina ulasmistir. Turkiye’de 1999-2010 yillarinda, su urunleri yetistiriciligi ile balik yemi uretimi arasinda pozitif bir korelasyon (r = % 91,6) oldugu, ayni surecte balik yetistiriciliginin balik yemi uretimine gore regresyonun ise 0,60 oldugu, ayrica 2007–2010 yillarinda ise bu uretimler yaklasik olarak bas-basa gitmeye baslamis.

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The effects of music on fish growth and stress.

B. Sağlamti̇mur Orhan Demir

One of the ways to decrease stress in fish growing systems is to grow fish with music. The effects of music on people has been studied for ages; furthermore, the effects of music on terrestrial animals have been studied since the last century. In relation with the increase of scientific studies on hearing and acousto lateralis systems, there have been efforts to use music in order to have a more suitable adaptation in aquatic environments although these efforts are few in number.

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THE INHIBITORY SITUATIONAL ANALYSIS OF SOME FEED INGREDIENTS FOR MEAGRE, Argyrosomus regius (Asso 1801) LARVAE AND EVALUATION FOR DIET FORMULATIONS

AQUATIC RESEARCH (2019)

Gürkan Diken Orhan Demir Mehmet Naz

Meagre, Argyrosomus regius (Asso 1801) is an important alternative species in aquaculture.The in vitro assay provides practical assessments for the evaluation of feed ingredients.In this study, the inhibition degrees of feed ingredients (fish meal-FM, fish hydrolysate-FH, krill meal-KM, soybean meal-SM, wheat gluten-WG, corn gluten-CG and sunflower meal-SF) on protease activities of meagre larvae were determined.Larvae were sampled from the first day of opening the mouth (3 days after hatching-DAH) until the end of the weaning (32 DAH) from the Egemar Hatchery (Aydın-Turkey).Larvae of the total length were measured as 3.19 ±0.02-21.61±0.22 mm and weights were calculated as 0.53 ±0.02-118.00±1.09 mg at 3 and 32 DAH, respectively.Protease activities of larvae were the lowest as 5.95 ±0.60 U/mg protein (15 DAH) and the highest as 211.21 ±12.56 U/mg protein (7 DAH), respectively (P<0.05).The lowest inhibitions degrees of feed ingredients were observed at 15 DAH except for SF.The use of FH in the diet formulations of meagre larvae should be paid attention.While CG and SF are advised, SM does not seem to be suitable.

Hatchery

Corn gluten meal

10.3153/ar19006

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Effects of the use of feeds containing phytase enzyme from different protein sources on nitrogen and phosphorus discharge of rainbow trout (Oncorhynchus mykiss) juveniles

Iranian journal of fisheries science/‏‫‬‭Iranian journal of fisheries science (2020)

Orhan Demir Adil Yılayaz

In this study, the effects of diets (D1-3) including fish meal (FM), hazelnut meal (HM), soybean meal (SM), and phytase enzyme [- (0),+(1000 FTU)] in varying rates (D1-, D1+; D2-, D2+; D3-, D3+) were examined on nitrogen and phosphorus discharge based on the nutrition of rainbow trout (Oncorhynchus mykiss) juveniles. The study was conducted in tanks with 3 replicates for each group. In this trial, it was found that the differences among total-particle nitrogen, particle phosphorus, and total solid waste values released from the rainbow trout groups were insignificant; however, the differences between the values of total-dissolved phosphorus and dissolved nitrogen released were found significant (p 0.05). Moreover, although there were increases in the total and dissolved phosphorus and dissolved nitrogen discharge in all groups fed with diets including phytase, the particle phosphorus discharge decreased (p<0.05). Based on the decrease in the amounts of hazelnut meal and soybean meal used in the diets, it was determined that there was a decrease in the amount of dissolved phosphorus released to the environment; besides, it was determined that it led to an increase in the amount of released dissolved phosphorus in all groups fed with diets containing phytase enzyme (p<0.05).

Phytase

10.22092/ijfs.2019.118085

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The effects of different feeding times and diets on the whole body fatty acid composition of goldfish (Carassius auratus) larvae

Figshare (2019)

Orhan Demir Süleyman SARIGÖZ

Abstract In this study, the effects on whole body fatty acid levels of goldfish larvae (Carassius auratus) caused by different feeding times T(1–4) and diets D(1–3) were investigated during the early larval stages which is the first 15 days’ of feeding. Fatty acid levels for all experimental groups were found to be: 1.54 to 3.45% for myristic acid, 17.57 to 22.68% for palmitic acid, 28.81 to 41.16% for total saturated fatty acids-∑SFA, 2.37 to 4.04% for palmitoleic acid, 13.79 to 39.21% for oleic acid, 22.60 to 47.59% for total monounsaturated fatty acids-∑MUFA, 3.62 to 7.69% for linolenic acid-ALA, 0.35 to 1.11% for arachidonic acid-ARA, 7.50 to 9.76% for total n-6 fatty acids-∑ n-6, 1.04 to 2.28% for linoleic acid-LA, 0.81 to 2.52% for eicosapentaenoic acid-EPA and 5.56 to 10.28% for docosahexaenoic acid-DHA. The total n-3 acids-∑ n-3, total polyunsaturated fatty acids-∑PUFA, PUFA/SFA ratio, ∑ n-6/∑ n-3 and DHA/EPA ratio of the experimental groups were determined to be 7.24 to 14.83%, 14.73 to 23.46%, 0.47 to 0.73, 0.58 to 1.04 and 3.21 to 8.7, respectively. In conclusion, the whole body fatty acid levels of goldfish larvae (Carassius auratus) determined in this study revealed the significant effects of different feeding times (T1–4) and different diets (D 1–3) using (P < 0.05).

Carassius auratus

Fish larvae

10.6084/m9.figshare.7773299

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The Inhibitory Effects and Positive Contributions of Live Foods on Protease Activities of Meagre, <em>Argyrosomus regius</em> (Asso 1801) Larvae <em>in vitro</em> Assay

Gürkan Diken Orhan Demir Mehmet Naz

The aim of this study was to gather preliminary data about the potential inhibitory effects and contributions of live foods used from 3 to 32 days after hatching (DAH) in routine feeding protocols on protease activities of meagre, Argyrosomus regius (Asso 1801) larvae, using in vitro techniques. Enriched rotifer, Artemia nauplii and Artemia metanauplii were tested in the present study. The highest values of protease activities of meagre larvae at 7 DAH in 2013 and 2014 years were established. The lowest values at 15 DAH in 2013 and at 20 DAH in 2014 were observed. Protease activities of enriched rotifer, Artemia nauplii, and Artemia metanauplii were 21.76±0.31, 36.00±1.48–29.33±0.93, and 416.44±19.7–403.53±11.85 U/mg protein, respectively (p< 0.05). The highest inhibitions of live foods were observed at 7 DAH. The positive contributions of live food Artemia metanauplii’s on protease activities of meagre larvae were significant (p< 0.05). The inhibitory effects and positive contributions of live foods on survival and growth rates of meagre larvae, should be taken into account in meagre and other marine fish larvae’s future studies. Cysteine protease activities of Artemia sp. should be investigated to provide the higher growth and survival ratio from the feeding protocols used in marine fish larvae.

Live food

10.20944/preprints201811.0150.v2

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Analysis of sarcoplamic proteins in natural populations of mountain trout (Salmo trutta macrostigma Dumeril, 1858) with SDS-PAGE

AFRICAN JOURNAL OF BIOTECHNOLOGY (2011)

Orhan Demir Ali Guuml nluuml Fahretti̇n Küçük skender Gulle

Molecular weights and band counts of sarcoplasmic proteins in four populations of Salmo trutta macrostigma living in Mediterranean region of Turkey were analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method and densitometric analysis. 13, 17, 14 and 15 sarcoplasmic protein bands were obtained, respectively from EAŸen Stream (I), Aksu Stream (II), Alara Stream (III) and AlakA±r Stream (IV) populations. Protein bands with molecular weights 210, 93 and 30 kDa were found solely in population II. 116 kDa bands were present in all populations but population I with density of bands with molecular weights over 97 kDa was smaller than all the other populations. Densitometric analysis of population IV showed that it differs from the three other poulations by having all protein bands except for 45 kDa band which were at low densities. Key words: Sarcoplazmic proteins, mountain trout, Salmo trutta macrostigma, SDS-PAGE, electrophoresis.

Brown trout

Molecular mass

Sodium dodecyl sulfate

10.5897/ajb.9000047

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Citations (2)

WHEN TO ADJUST SERUM CHLORIDE FOR ANION GAP CALCULATION?

European Journal of Internal Medicine (2011)

Mustafa Gezer Fatih Bulucu Kadir Öztürk Orhan Demir

Anion gap

Serum chloride

10.1016/s0953-6205(11)60288-4