ABSTRACT Received: Revised: Accepted: January 27, 2010 April 05, 2010 April 15, 2010 Key words: Brown trout Fish culture Larval development Reproductive features S. trutta macrostigma In this study, some reproductive features of wild brown trout ( Salmo trutta macrostigma Dumeril, 1858) populations in Aksu, Esen, Alara and Alakir streams in Turkey were examined and larval development was also investigated in two different culture conditions established on Esen Stream (Trial 1) and at the Research Unit of Egirdir Faculty of Fisheries at Suleyman Demirel University (Trial 2). The spawning time of brown trout in Aksu Stream in the second half of December was different from other localities, at the end of the January and in the first week of February. The gonadosomatic index values ranged between 17.5 and 19.8%, the mean egg diameters of females in all localities were between 3.51 and 3.78 mm (P<0.05). The incubation lasted for 370 and 390 day-degree (10.5-10.9 oC), and the yolk-sac of the larvae was absorbed in 28 and 32 days in trials 1 and 2, respectively. The mean live weight (LW), total length (TL) and mouth widths (MW) in start-fed larvae and those with absorbed yolk-sac in both trials showed non significant differences before the 25
This study aimed to examine the effects of six trial diets (containing different amounts of fish meal, hazelnut, and soybean meal protein, also without the enzyme phytase “0 FTU” and with “1000 FTU”) on the rainbow trout (Oncorhynchus mykiss) juveniles. The study was conducted as three replicates. Live weight gain, feed consumption, feed conversion ratio, specific growth ratio and the protein efficiency ratio of the trial groups were calculated as 52.8-64.3g, 64.1-72.1g/fish, 1.12-1.22, 1.42-1.6% and 1.79-2.0, respectively (P<0.05). The dietary factors did not produce a significant effect on the organ indexes, body chemical composition, digestibility ratios of diets in the fish (P>0.05). But phytase enzyme affected significantly the dietary phosphorus ratio and the digestible energy ratio (P<0.05). Dry matter, crude ash, crude lipid, crude protein and phosphorus components in the fish bodies were found to be 30.7-31.8%, 2.2-2.3%, 11.8-12.8%, 14.5-15.2% and 0.56-0.62%, respectively. In the trial group fishes, values of the total nitrogen and phosphorus consumption, gain and retention were calculated as 1139-1173 mg/kg versus 248-279 mg/kg, 315-350 mg/kg versus 73-82 mg/kg, and 27.2-30.6% versus 26-33%, based on the average daily weight. Generally, the interaction between factors was found to be statistically insignificant (P>0.05).
The determination of exogenous enzyme contributions in live food is important in larval feeding. This study investigated the potential inhibitory effects and contributions of live foods used from 3 to 32 days after hatching (DAH) on protease activities of meagre (Argyrosomus regius) larvae (ML), using in vitro techniques. Enriched rotifer (R), Artemia nauplii (A0), and Artemia metanauplii (A1) were tested. The highest values of protease activities of ML were determined to occur at 7 DAH for the years 2013 and 2014. The lowest values were observed at 15 DAH in 2013 and at 20 DAH in 2014. Protease activities of R, A0, and A1 were 21.76 ± 0.31, 36.00 ± 1.48–29.33 ± 0.93, and 416.44 ± 19.7–403.53 ± 11.85 U/mg protein, respectively (p < 0.05). The highest inhibitions of live foods were observed at 7 DAH. This situation was related to the inadequacy of protease contributions of live feeds, despite the highest protease value of larvae at 7 DAH (p < 0.05). The positive contributions of A1 live food on the protease activities of ML were significant (p < 0.05). The inhibitory effects and positive contributions of live foods on the survival and growth rates of ML should be taken into account for future studies considering the larvae of ML and other marine fish.
The aim of this study was to gather preliminary data about the potential inhibitory effects and contributions of live foods used from 3 to 32 days after hatching (DAH) in routine feeding protocols on protease activities of meagre, Argyrosomus regius (Asso 1801) larvae, using in vitro techniques. Enriched rotifer, Artemia nauplii and Artemia metanauplii were tested in the present study. The highest values of protease activities of meagre larvae at 7 DAH in 2013 and 2014 years were established. The lowest values at 15 DAH in 2013 and at 20 DAH in 2014 were observed. Protease activities of enriched rotifer, Artemia nauplii, and Artemia metanauplii were 21.76±0.31, 36.00±1.48–29.33±0.93, and 416.44±19.7–403.53±11.85 U/mg protein, respectively (p< 0.05). The highest inhibitions of live foods were observed at 7 DAH. The positive contributions of live food Artemia metanauplii’s on protease activities of meagre larvae were significant (p< 0.05). The inhibitory effects and positive contributions of live foods on survival and growth rates of meagre larvae, should be taken into account in meagre and other marine fish larvae’s future studies. Cysteine protease activities of Artemia sp. should be investigated to provide the higher growth and survival ratio from the feeding protocols used in marine fish larvae.
The present study is designed to report the potential effects of commercial feeding protocol on protease activities and cortisol stress responses of meagre (Argyrosomus regius) from fertilized eggs to 45 days after hatching (DAH). The inhibition effects of six commercial diets (Gemma Micro 150 (100-200µ), Caviar (200-300µ), Caviar (300-500µ), Perla Larva Proactive 4.0 (300-500µ), Perla MP-S(700 µ) and Perla Plus 3.0(400-800 µ)) and protease contributions of live foods (enriched rotifer, Artemia nauplii and Artemia metanauplii) were tested in the study. Samples of meagre were collected from fertilized eggs to 45 DAH. All analyses were carried out in triplicates.The differences observed in the total length and weight values were statistically significant (p< 0.05). The lowest and highest total length values were 3.06±0.23 mm (3 DAH) mm and 33.28±0.68 mm (45 DAH), respectively. The lowest and highest weight values were 0.53±0.01 mg (3 DAH) and 467.67±1.47 mg (45 DAH), respectively. The differences in protease activities of meagre larvae were statistically significant (p< 0.05). The highest and lowest protease activities of meagre larvae were 477.13±39.30 U/mg protein (3 DAH) and 10.05±1.16 U/mg protein (20 DAH), respectively. The digestive proteases of meagre larvae showed the greatest sensitivity to protease inhibitors present in Gemma Micro 150 (100-200µ). Fertilized eggs of meagre had low cortisol level (0.68 ng/g). Cortisol values increased rapidly up to hatching (2.43±0.06 ng/g) and followed by a decrease in prelarval stage (1.80±0.01 ng/g) and then remained relatively until before mouth opening (1.76±0.03 ng/g). From 3 to 45 DAH, the highest and lowest cortisol values of meagre larvae were 6.13±0.18 ng/g (15 DAH) and 1.98±0.17 ng/g (30 DAH) respectively. In conclusion, the presence of cortisol in fertilized eggs of Argyrosomus regius confirms that they are maternal origin. Perla MP-S(700 µ) and Perla Plus 3.0(400-800 µ)) are suggested due to good performances from 3 to 45 DAH but not Gemma Micro 150 (100-200µ). Caviar (200-300µ) and Caviar (300-500µ) are the moderately advisable as commercial diets in the feeding protocol of meagre larvae except for more inhibitions than 50%.
The aim of this study is to investigate the possibilities of using the meteorological data obtained from the public meteorology station in determining the effect of air temperature and relative humidity on milk yield in Black and White dairy cattle raised in Diyarbakır province of Turkey. Records on daily milk yield obtained from a commercial farm in Diyarbakır province were used in the study. Meteorological records containing daily maximum and minimum temperature and daily maximum and minimum humidity data were obtained from nearest public weather station in Diyarbakır province. Data set related to milk yields includes 46 438 daily records during five different lactations of 185 healthy Black and White dairy cattle and information of some environmental factors of the animals. In the study, five different temperature-humidity index (THI) types consisting of different combinations of daily maximum, minimum and average temperature and humidity values were considered to evaluate the effect of heat stress on milk production.
This study confirmed that weather stations located away from the farms contain useful information for research on heat stress in dairy cows. Using the combination of maximum daily air temperature and minimum daily humidity in THI formula performed better than other THI types in quantifying the heat stress in this study. This combination was less affected by other environmental factors and the results obtained from this combination seems to be more biologically meaningful. According to this, dairy cattle were exposed to heat stress for 160 days between May and October in a year. In this period, the losses of milk production were 1518.85 and 0.73 kg for per cow per year and per unit THI increase, respectively.
In Turkey, knowledge about production aquaculture and fish feed are notice to formal statics recently. Development of science researc and investment lead to increasing rapidly in production of aquaculture and fish feed sectors. Firstly, in 1999 name of “fish feed” was recorded in statics of Turkey (38425 tons) and quantity of fish feed was increased to 70153 tons in 2006. Knowledges about aquaculture was recorded to the first time in 1986 and quantity of production was 3075 tons. In 2005, production of aquaculture was increased 118277tons in other words total aquaculture production of Turkey was reached share 22 %.