Lishan Lei

Fujian Medical University

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Xiaojing Huang

Fujian Medical University

Zhiyu Cai

Fujian Medical University

Yanhuang Wang

Fujian Medical University

Yijun Li

Fujian Medical University

Huan Ge

Xiangya Hospital Central South University

Yuemin Chen

Fujian Medical University

Shan Huang

Jinggangshan University

Ronghui Zhou

Fudan University

Chengfei Zhang

Beijing University of Civil Engineering and Architecture

Fang Wang

Zhejiang University of Technology

Cooperative Institutions

Fujian Medical University

Division of Undergraduate Education

American Association of Endodontists

Clinical Research Management

Peking University

Sichuan University

Chinese Academy of Sciences

Shanghai Jiao Tong University

Universidade de São Paulo

Université Toulouse III - Paul Sabatier

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Pretreatment of Root Canal with Photodynamic Therapy Facilitates Adhesion, Viability and Differentiation of Stem Cells of the Apical Papilla

Photochemistry and Photobiology (2020)

Yijun Li Huan Ge Lixuan Wu Lishan Lei Yanhuang Wang

Abstract This study was to test the hypothesis that root canal pretreated with photodynamic therapy (PDT) would promote stem cells from the apical papilla (SCAP) adhesion, proliferation and differentiation without affecting smear layer removal and microhardness of root canal. Standardized root canals were randomized into four groups ( n = 30/group): (1) sodium hypochlorite(NaOCl) group, (2) NaOCl + ethylene diaminetetraacetic acid (EDTA) group, (3) NaOCl + PDT group, (4) NaOCl + EDTA + PDT group. After treatments, smear layer removal and microhardness of root canal were evaluated. SCAP with hydroxyapatite‐based scaffolds were seeded into root canals for 7 days. SCAP adhesion was observed by scanning electron microscope (SEM), and viable cells were calculated by CellTiter‐Glo Luminescent kit. Platelet‐derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) expression of SCAP were evaluated by Quantitative Reverse Transcriptase‐Polymerase Chain Reaction. There was no significant difference in the smear layer removal and microhardness of root dentin between the groups with and without PDT treatment ( P > 0.05). SCAP with elongated cytoplasmic processes and cell–cell contact were observed on the dentin surfaces treated with PDT. Elevated cell viability, PDGF and VEGF expression were found in root canal treated with PDT ( P < 0.05). Under the experimental conditions, PDT could provide positive microenvironment for SCAP growth.

Sodium hypochlorite

Viability assay

Smear layer

10.1111/php.13240

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Citations (6)

[Effect of the self-etching adhesives system on human pulp fibroblast].

Ming Zhang Feng Yan Xiaojing Huang Lishan Lei Bi-qiong Zheng

OBJECTIVE To compare and evaluate the biocompatibility of three kinds of dentin bonding agents Xeno III (XO), Adper Prompt (AP), Single bond2 (SB) through cell culture in vitro. METHODS Three kinds of dentin bonding agents (XO, AP, SB) were applied on the surface of the dental slices which were 5.0 mm in diameter and 0.5 mm in depth. By immersing the slices into the DMEM culture medium, the maceration extracts were obtained. Normal dental pulps of teenagers were collected and human pulp fibroblast was cultured using tissue explant method. The fifth generation pulp cells were exposed to culture medium containing different concentrations of maceration extracts (100.0%, 50.0%, 25.0%, 12.5%) for 24, 72, 120 h. At last, MTT method was used to evaluate the cytotoxicity of the dentin bonding agents on human pulp fibroblast. RESULTS The results showed that all three kinds of dentin bonding systems had cytotoxicity to human pulp fibroblast in different degree in vitro. The cytotoxicity of XO and AP was less than SB. The difference was statistically significant (P<0.05). CONCLUSION The results of cell culture in vitro indicated that total-etching adhesives system has more irritation to pulp than self-etching adhesives system.

Maceration (sewage)

Biocompatibility

MTT assay

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Citations (1)

Hydrogen peroxide potentiates antimicrobial photodynamic therapy in eliminating Candida albicans and Streptococcus mutans dual-species biofilm from denture base

Photodiagnosis and Photodynamic Therapy (2021)

Yijun Li Jingyun Du Shan Huang Shaofeng Wang Yanhuang Wang

10.1016/j.pdpdt.2021.102691

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Citations (26)

Sonic-assisted antibacterial photodynamic therapy: a strategy for enhancing lateral canal disinfection

BMC Oral Health (2024)

Yanhuang Wang Lishan Lei Jing Huang Zhiyu Cai Xiaojing Huang

Abstract Background Bacterial infections in lateral canals pose challenges for root canal treatment. This in vitro study aims to evaluate the antibacterial efficacy of sonic-assisted methylene blue mediated antimicrobial photodynamic therapy (MB-aPDT) against Enterococcus faecalis ( E. faecalis ) in infected lateral canals. Methods Sixty-five premolars infected with E. faecalis in lateral canals were randomly divided into five groups (n = 13) and treated with : (1) 5.25% NaOCl (positive control); (2) Saline (negative control); (3) Sonic-assisted MB-aPDT; (4) 3% NaOCl + MB-aPDT; (5) 3% NaOCl + sonic-assisted MB-aPDT, respectively. The antibacterial efficacy was evaluated by the colony- counting method (CCM) and scanning electronic microscope (SEM). Results Both 5.25% NaOCl and the 3% NaOCl + sonic-assisted MB-aPDT exhibited the most effective while comparable antibacterial effects without significant statistical difference (P > 0.05). Furthermore, the antibacterial effect of the 3% NaOCl + MB-aPDT group was significantly higher compared to that of the sonic-assisted MB-aPDT group (P < 0.05). The SEM results demonstrated notable morphological alterations in E. faecalis across all experimental groups, except for the negative control group. Conclusion The concentration of NaOCl can be reduced to a safe level while preserving its antibacterial efficacy through the synergism with the sonic-assisted MB-aPDT in this study.

Enterococcus faecalis

Methylene blue

10.1186/s12903-023-03801-6

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Citations (1)

Histologic and Immunohistochemical Findings of a Human Immature Permanent Tooth with Apical Periodontitis after Regenerative Endodontic Treatment

Journal of Endodontics (2015)

Lishan Lei Yuemin Chen Ronghui Zhou Xiaojing Huang Zhiyu Cai

Apical foramen

Human tooth

Dental papilla

10.1016/j.joen.2015.03.012

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Citations (103)

The efficacy of platelet-rich fibrin as a scaffold in regenerative endodontic treatment: a retrospective controlled cohort study

BMC Oral Health (2018)

Hongbing Lv Yuemin Chen Zhiyu Cai Lishan Lei Ming Zhang

Platelet-rich fibrin

10.1186/s12903-018-0598-z

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Citations (48)

Disinfection of Cariogenic Pathogens in Planktonic Lifestyle, Biofilm and Carious Dentine with Antimicrobial Photodynamic Therapy

Photochemistry and Photobiology (2019)

Minjing Wu Xu Li Zhiyu Cai Shan Huang Yijun Li

Antimicrobial photodynamic therapy (aPDT) has been recommended for clinical application. Its antibacterial effect on bacteria remained in dentinal tubule was seldom investigated. Here, we evaluated the antibacterial effects of aPDT on Streptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus) in planktonic lifestyle, biofilm and carious dentine. Mono-species biofilms or dentinal caries formed on human dentine slices or slabs. Bacterial suspension, biofilms and dentine caries were treated with 0.1 mg mL-1 toluidine Blue O followed by irradiation with a light emission diode (λ - 635 ± 10 nm; 500 mW; 31.5 J cm-2 ; 60 s) and 0.12% chlorhexidine (CHX), respectively. Residual bacteria were determined by microbial culture analysis and scanning electron microscopy (SEM). One-way analysis of variance (ANOVA) was performed to detect the significance of the variables. Both treatments significantly reduced the number of L. acidophilus in planktonic state, biofilm and carious dentine (P < 0.05). For S. mutans, CHX was only bactericidal against suspension (P < 0.05), while aPDT was effective on both suspension and biofilm (P < 0.05) while not for dentin caries (P > 0.05). Under the experimental conditions assessed, aPDT could be an alternative disinfection method for superficial layer of caries cavity. Its disinfection on bacteria in dentinal tubule of deep layer was deficient.

10.1111/php.13161

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Citations (17)

Effect of combined application of tea polyphenols and grapefruit-seed extract on growth and acid production of Streptococcus mutans

Disan Junyi Daxue Xuebao (2011)

Huang XiaoJing LU Guo-ying Jiandang Liu Shan Jiang Lishan Lei

Citrus paradisi

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Disinfect Porphyromonas gingivalis Biofilm on Titanium Surface with Combined Application of Chlorhexidine and Antimicrobial Photodynamic Therapy

Photochemistry and Photobiology (2018)

Zhiyu Cai Yijun Li Yanhuang Wang Shuai Chen Shan Jiang

Abstract Various antimicrobial modalities have been proposed to treat peri‐implantitis but resulted in limited outcomes. The aim of this in vitro study was to evaluate the disinfection efficacy of combined application of chlorhexidine digluconate ( CHX ) and antimicrobial photodynamic therapy ( aPDT ) of titanium surfaces previously contaminated with Porphyromonas gingivalis biofilm. P. gingivalis biofilms were grown on 32 polished and 32 sandblasted large‐grit acid‐etched ( SLA ) titanium surfaces. Titanium disks were allocated into four groups as follows: (1) immersed in phosphate‐buffered saline ( PBS ), (2) immersed in 0.2% CHX , (3) application of aPDT and (4) immersed in 0.2% CHX and subsequent aPDT . Residual bacteria were determined by microbial culture analysis and by scanning electron microscopy ( SEM ) and confocal laser scanning microscopy ( CLSM ) imaging. Combination protocol ( CHX + aPDT ) was the most effective in eradicating P. gingivalis ( P < 0.05) on both polished and SLA surfaces. There was no significant difference in the number of remaining P. gingivalis between polished titanium disks and the SLA ones in four groups ( P > 0.05). Under the limitation of this study, combined technique of preceding application of CHX and subsequent aPDT was shown to be an efficient method in reducing P. gingivalis numbers in both polished and SLA titanium surfaces.

Peri-implantitis

10.1111/php.13060

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Citations (15)

Antimicrobial effects of photodynamic therapy with antiseptics on Staphylococcus aureus biofilm on titanium surface

Photodiagnosis and Photodynamic Therapy (2019)

Zhiyu Cai Yijun Li Yanhuang Wang Shuai Chen Shan Jiang

Antiseptic

Human decontamination

10.1016/j.pdpdt.2019.01.024

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Citations (33)