Objective:To study the levels of hormones and growth and development of follicles after vitrification cryopreservation of rabbit ovarian tissues,ectopic subcutaneous transplantation.Methods:36 nude rats were divided into control group,non-frozen group,frozen group and ovariectomized group randomly;non-frozen and frozen ovarian tissues were ectopically transplanted into subcutaneous tissues of neck in the nude rats,the estrous cycles were observed on the fifth days after transplantation,then the serum level of estrogen in the rats was measured on the thirty-fifth day after transplantation,wet weights and histological morphologies of uterus in the four groups were observed and compared.Results:Alive ovarian grafts were observed in non-frozen group and frozen group,follicles at different developmental stages as well as interstitial glands and corpora luteum existed in ovaries;during estrous cycles,there was no significant difference in serum estrogen level between non-frozen group,frozen group and control group(P0.05),and the serum estrogen levels in the above mentioned groups were significantly higher than that in ovariectomized group.Conclusion:Vitrification cryopreservation can preserve the structure and function of ovarian tissues effectively,frozen-thawed ovaries have the similar physiological activity as fresh ovarian cortex;ectopic subcutaneous transplantation of frozen ovarian cortex can survive,grow and maintain reproductive endocrine function.
The catechol-O-methyltransferase (COMT) Val158Met polymorphism has been reported to be implicated in generalized anxiety disorder (GAD) as well as the treatment response to antidepressants in patients with GAD, but the findings are inconsistent. In this study, we explore the association among COMT, GAD, and the antidepressant response in the Chinese Han population. One hundred and two patients with GAD and 120 healthy controls (HC) were recruited. All the patients were treated with escitalopram or venlafaxine for 8 weeks. The Hamilton Rating Scale for Anxiety (HAMA) was used to assess the treatment response. All the participants were genotyped for the COMT Val158Met polymorphism using the polymerase chain reaction method. No significant differences in the frequency of the COMT rs4680 polymorphism were found between the GAD and HC groups, or between patients with different genders. Further, we found no significant correlation between the COMT rs4680 polymorphism, gender, and the antidepressant treatment outcomes after eight weeks in the GAD patients. This study indicated that the COMT rs4680 genotype might not be related to GAD or to the genders of the GAD patients, nor did it have any effect on the antidepressant therapeutic response in the GAD patients. Even so, our research will be helpful by providing guidance and direction for future, more in depth, research.
Vaccination plays a key role in preventing morbidity and mortality caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We aimed to evaluate the safety and immunogenicity of a SARS-CoV-2 messenger ribonucleic acid (mRNA) vaccine SYS6006. In the two randomized, observer-blinded, placebo-controlled phase 1 trials, 40 adult participants aged 18–59 years and 40 elderly participants aged 60 years or more were randomized to receive two doses of SYS6006 or placebo (saline). Adverse events (AEs) were collected through 30 days post the second vaccination. Immunogenicity was assessed by live-virus neutralizing antibody (Nab), spike protein (S1) binding antibody (S1-IgG), and cellular immunity. The result showed that 7/15, 9/15 and 4/10 adult participants, and 9/15, 8/15 and 4/10 elderly participants reported at least one AE in the 20-µg, 30-µg and placebo groups, respectively. Most AEs were grade 1. Injection-site pain was the most common AE. Two adults and one elder reported fever. No vaccination-related serious AE was reported. SYS6006 elicited wild-type Nab response with a peak geometric mean titer of 232.1 and 130.6 (adults), and 48.7 and 66.7 (elders), in the 20-µg and 30-µg groups, respectively. SYS6006 induced moderate-to-robust Nab response against Delta, and slight Nab response against Omicron BA.2 and BA.5. Robust IgG response against wild type and BA.2 was observed. Cellular immune response was induced. In conclusion, two-dose primary vaccination with SYS6006 demonstrated good safety and immunogenicity during a follow-up period of 51 days in immunologically naive population aged 18 years or more. (Trial registry: Chictr.org.cn ChiCTR2200059103 and ChiCTR2200059104)
PURPOSE:To investigate the association of human leukocytes antigen HLAⅡ-DRB1 alleles between Han Chinese and Uygur people with oral lichen planus(OLP) in Xinjiang Uygur Autonomous Region.METHODS:The allele frequency of HLAⅡ-DRB1 was detected in 34 OLP patients vs 123 normal controls of Han Chinese group and 14 OLP patients vs 42 normal controls of Uygur people group by polymerase chain reaction-sequence specific primers(PCR-SSP) method.The data was analyzed with SPSS16.0 software package for Chi-square test and Fisher’s exact test.RESULTS:The allele frequency of HLAⅡ-DRB1*11 was significantly higher(23.53% vs 9.76%),however,HLAⅡ-DRB1*12 was substantially lower(5.88% vs 22.76%) in OLP patients than that in normal controls in Han Chinese group,P0.05.The allele frequency of HLAⅡ-DRB1*140 was significantly higher(50.00% vs 9.52%) than that in normal controls in Uygur people group,P0.05.CONCLUSIONS:HLAⅡ-DRB1*11 alleles could be the susceptible genes for OLP in Han Chinese in Xinjiang Uygur Autonomous Region,however,HLAⅡ-DRB1*12 alleles might be the protective gene for OLP in Han Chinese in Xinjiang Uygur Autonomous Region.HLAⅡ-DRB1*14 could be the susceptible genes for OLP in Uygur people in Xinjiang Uygur Autonomous Region.
Objective To study the pathogenesis of acute focal ischemia cerebral injury.Methods Establishing the animal model of focal cerebral ischemia by using suture method in mature Wistar rats, detecting the brain water content and pathological changes as well as the activity of malonaldehyde (MDA), superoxides dismutase (SOD), nitric oxide synthase (NOS), glutathione peroxidase (GSH Px), catalase (CAT) in brain tissue after two hours of cerebral ischemia.Results The activity of MDA,SOD, and NOS was higher, but GSH, Px, and CAT was lower in ischemic brain tissues than control ones, concomitanting the increase of brain water content and evident pathological changes.Conclusion Oxygen free radicals have been found to participate in the pathological process of acute focal ischemic cerebral injury.
Objective
To study the clinicopathological significance and malignant behavior of human colorectal cancer (CRC) with the expression of neuronal pentraxin 2 (NPTX2).
Methods
NPTX2 expression in 101 CRC and paired adjacent normal tissues and its clinicopathological significance were detected by immunohistochemistry (IHC). Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blotting were used to examine NPTX2 protein and mRNA levels in 18 paired CRC and adjuvant normal tissues. Finally, small interfering RNA (siRNA) was used to detect the effect of NPTX2 silencing on CRC cell invasion and migration and its possible mechanism.
Results
NPTX2 expression in CRC tissues was significantly higher than that in non-cancerous tissues (Z=-6.083, P<0.01). NPTX2 overexpression was positively associated with tumor size (χ2=7.324, P<0.01), T stage (χ2=5.811, P<0.05) and TNM stage (χ2=4.791, P<0.05). Moreover, patients with NPTX2 high expression had a worse prognosis (P<0.01). RT-qPCR and Western blotting showed that NPTX2 mRNA and protein levels in 18 CRC tissues were significantly higher than those in paired normal tissues (t=2.612, P<0.05; t=2.533, P<0.05). Meanwhile, NPTX2 silence inhibited cell invasion and migration in SW480 cell lines, along with the increase of E-cadherin and decrease of N-cadherin and snail1.
Conclusion
NPTX2 overexpression promotes the development and progression of human colorectal cancer.
Key words:
Colorectal cancer; Neuronal pentraxin 2; Invasion; Migration; Prognosis
Objective
To investigate the effect of baclofen combined with adenosine cobalt amine on serum inflammatory factors and stress response in patients with trigeminal neuralgia.
Methods
88 patients with trigeminal neuralgia treated in our hospital from February, 2016 to February, 2019 were randomly divided into a control group and an observation group, 44 cases for each group. The control group were treated with baclofenac tablets, while the observation group with baclofenac tablets and adenosine cobalt amine tablets. The cycle of both groups was 3 months. The serum inflammatory factors and stress response were compared between the two groups before and after the treatment.
Results
After 3 months’ treatment, the serum levels of IL-6 ( IL-6, TNF- α, IL-1 β, and malondialdehyde (MDA) were lower and the serum levels of GSH-Px and SOD were significantly higher in the observation group than in the control group (all P<0.05).
Conclusion
Baclofen combined with adenosine cobalt amine can effectively treat patients with trigeminal neuralgia and reduce the levels of serum inflammatory factors and stress response, which is worth being popularized.
Key words:
Baclofen; Adenosine cobalt amine; Trigeminal neuralgia; Serum inflammatory factors; Stress response
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