Nasopharyngeal carcinoma (NPC), is an Epstein-Barr virus (EBV) associated malignancy most common in Southern China and Southeast Asia. In southern China, it is one of the major causes of cancer-related death. Despite improvement in radiotherapy and chemotherapy techniques, locoregional recurrence and distant metastasis remains the major causes for failure of treatment in NPC patients. Therefore, finding new specific drug targets for treatment interventions are urgently needed. Here, we report three potential Z LMP1-C affibody molecules (Z LMP1-C 15, Z LMP1-C 114 and Z LMP1-C 277) that showed specific binding interactions for recombinant and native EBV LMP1 as determined by epitope mapping, co-localization and co-immunoprecipitation assays. The Z LMP1-C affibody molecules exhibited high antitumor effects on EBV-positive NPC cell lines and displayed minimal cytotoxicity towards EBV-negative NPC cell line. Moreover, Z LMP1-C 277 showed higher antitumor efficacy than Z LMP1-C 15 and Z LMP1-C 114 affibody molecules. The ability of Z LMP1-C 277 decrease the phosphorylation levels of up-stream activator phospho-Raf-1 (Ser338) , phospho-MEK1/2 (Ser217/Ser221) , phospho-ERK1/2 (Thr202/Thr204) , thereby leading to downstream suppression of phospho-p90RSK (Ser380) and transcription factor c-Fos. Importantly, tumor growth was reduced in tumor-bearing mice treated with Z LMP1-C 277 and caused no apparent toxicity. Taken together, our findings provide evidence that Z LMP1-C 277 as a promising therapeutic agent in EBV-associated NPC.
1-Alkyl-3-methylimidazolium chloride (CnmimCl) can be used to construct bilayer CnmimCl stabilized magnetic fluids, and subsequently magnetic mesoporous silica can be prepared by using the C16mimCl as template in the magnetic fluids.
Accurate detection of copper ions is crucial since it is an essential trace element. Carbon quantum dots (CQDs) were prepared from apricot leaves using a hydrothermal method. These CQDs, which generated a reference signal, were combined with glutathione-bound gold nanoclusters (GSH-AuNCs) synthesized by the reduction of chloroauric acid to form a ratiometric fluorescent probe. The probe exhibited fluorescence emission at 450 nm and 568 nm. The determination of Cu(II) was performed through visual analysis of the color change and the intensity ratio (I450/I568) of the emission peaks. Orange fluorescent GSH-AuNCs responded to Cu(II) with fluorescence, and the blue fluorescent CQDs were chemically inert. The probes changed color from pink to purple to blue in response to different concentrations of Cu(II). The linear range of the fluorescence probe for Cu(II) was 0 to 120 µmol/L, and the limit of detection (LOD) was 0.65 µmol/L. This study has demonstrated that the probe provided good selectivity for detecting Cu(II) in water and has potential practical application.
Objective To investigate the effects of inhaled low dose nitric oxide(NO)on lung ischemia-reperfusion injury during flush and delayed 10 min after reperfusion.Methods Sixty health a- dult male Sprague-Dawley rats were randomly allocated to the control and the NO group.Before the donor lung was harvested,the right hilus was clipped for 5 min(clipping test),then blood sample was collected from carotid artery for arterial blood gas analysis as baseline.Lung transplantation was per- formed in a“cuff-like”vessel anastomosis technique.Dynamic compliance(Cdyn)and resistance of airway(Raw)were monitored before operation(baseline)and after 2-h reperfusion.The graft's gas exchange and oxygenation were assessed by“clipping test”after 2-h reperfusion.The lung graft was harvested for measuring wet/dry weight ratio(W/D),the activity of myeloperoxidase(MPO)and in- ducible nitric oxide synthase(iNOS),the content of malonyldialdehyde(MDA),and the expression of iNOS gene and protein.Results After 2-h reperfusion,compared to the control group,PaO_2/FiO_2, OI,and Qs/Qt were improved significantly in the NO group(277±91 vs.157±47,P＜0.01;2.67±0.89 vs.4.72±1.48,P＜0.01;21.1±4.57 vs.27.1±2.37,P＜0.01,respectively).The activi- ties of MPO were significantly reduced in NO group(1.80±0.46 vs 3.08±0.65 U/g tissue,P＜0.01).The content of MDA in the lung tissue of NO group was significantly higher than that of the control group(34.8±7.9 vs.20.0±11.2 nmol/mg protein,P＜0.05).Inflammatory cell infiltration was also significantly reduced(P＜0.05).The expression of iNOS gene and protein in the lung tissue of NO group was significantly lower than that of the control group.The activities of iNOS were also significantly reduced in NO group(10.6±10.2 vs 97.8±82.2 nmol·g~(-1)·min~(-1),P＜0.05).The im- munohistochemical positive staining of iNOS was localized in the alveolar epithelial cells and the in- flammatory cells infiltrated in the alveolar spaces and mesenchymal tissue.But there were no signifi- cant differences between two groups in Cdyn,Raw and W/D ratio.Conclusion Inhaled low dose NO might mitigate the intrapulmonary shunt,prevent neutrophil sequestration,inhibit the expression of iNOS gene and protein in isograft,thereby ameliorate ischemia-reperfusion injury and improve the ox- ygenation of the graft.
Abstract The significant effects of the heterogeneous structures of graphene and SiC have recently beendemonstrated for improving the performances of lithium‐ion batteries (LIBs), but the synthesis remains a grand challenge, owing to the rather high reaction temperatures (>1200 °C) of SiC crystallization. Herein, we present the design and synthesis of graphene/SiC composite nanosheets by using soda papermaking black liquor (SPBL) as a raw material through the in situ thermochemical method at 800 °C. The graphene/SiC composite has a Brunauer‐Emmett‐Teller surface area of 199.7 m 2 g −1 and exhibited a reversible capacity of 1044 mAh g −1 at 100 mA g −1 when used as a LIB anode. In addition, this anode retained a stable capacity of 230 mAh g −1 at a current density of 1 A g −1 after 1000 cycles. Even at a high current density of 10 A g −1 , it could still deliver a capacity of 122 mAh g −1 after 100 cycles. The superior cycle stability of graphene/SiC nanosheets is attributable to the unique nanocomposite structure. More importantly, this can effectively translate and utilize SPBL in the synthesis of high‐performance electrode materials, which greatly reduces the load of SPBL on our environment.
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